TABLE 2

Plasmids

NameDescriptionSource
pARB11.3-kb EcoRI-SalI fragment carrying the GAL1-zymocin γ-subunit gene fusion in YIplac211Butler et al. (1994)
pARB74.8-kb EcoRI-SalI fragment from pARB106 in YEplac181This study
pARB153.5-kb SalI fragment ftom pARB106 in YEplac181This study
pARB188.2-kb EcoRI-SalI fragment carrying SAP155 in YCplac111This study
pARB198.2-kb EcoRI-SalI fragment carrying SAP155 in YEplac181This study
pARB225.9-kb EcoRI-SalI fragment of pARB100 in YCplac111This study
pARB236.5-kb EcoRI-SalI fragment of pARB100 in YEplac181This study
pARB313.0-kb PvuII fragment of pAB106 in YEplac181This study
pARB363.8-kb PvuII fragment of pAB106 in YEplac181This study
pARB1005.25-kb fragment carrying GRX3/tRNAgln in pMA3aThis study
pARB1068.4-kb fragment carrying SAP155 in pMA3aThis study
CB337SIS2 in YEp24Kim Arndt
CB1317ADH1-CLN2 in LEU2-2μKim Arndt
CB1418ADH1-PCL1 in LEU2-CENKim Arndt
CB2606SAP190 in YEp24Kim Arndt
CB2643SAP155 in YEp24Kim Arndt
CB2645sap155fs in YEp24 (frameshift mutation in SAP155)Kim Arndt
CB2819SAP185 in YEp24Kim Arndt
CB2925SAP4 in YEp24Kim Arndt
pDG63.3-kb Psp1406I fragment of SAP155 in the ClaI site of pBluescript II SK+This study
pDG83.8-kb XbaI-Psp1406I fragment of SAP155 in YEplac181This study
pDG11, 12sap155::HIS3 versions of pDG8This study
pDG140.62-kb XhoI-BamHI fragment of pARB100 in YEplac181 (SalI-BamHI) carrying tRNAglnThis study
pDG151.4-kb SpeI-XhoI fragment of pARB100 in YEplac181 (XbaI-SalI) carrying GRX3 and tRNAglnThis study
pDJ141.8-kb EcoRI-PstI fragment carrying TAP42 in YEplac195This study
pJHW272.9-kb DraI fragment carrying KTI12 in YEplac181Butler et al. (1994)
pLF161.45-kb EcoRI-HindIII fragment of pARB1 (carrying the GAL1-zymocin γ-subunit gene fusion) in YCplac111This study
YCplac33CEN4 ARS1 URA3 yeast shuttle vectorGietz and Sugino (1988)
YCplac111CEN4 ARS1 LEU2 yeast shuttle vectorGietz and Sugino (1988)
YEp24URA3 yeast shuttle vectorBotstein et al. (1979)
YEplac181LEU2 yeast shuttle vectorGietz and Sugino (1988)
YEplac195URA3 yeast shuttle vectorGietz and Sugino (1988)
pYF11.9-kb HindIII-BamHI fragment carrying Embedded Image in YEplac181Butler et al. (1994)