TABLE 4

Gene conversion data for crosses of ascospore color mutants to wild types from the south- or north-facing slopes of Evolution Canyon

Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR2.3 (SG1 from S3)SR4.2 (SG1 from S2)SR1.4 (SG1 from N5)SR5.3 (SG1 from N7)
Sw18.2, selfed generation 1 strain from the original S3 strain
8:07213
0:8a6223
7:110467
1:75433
6:2, 6:2(2)1146211057
2:6, 2:6(2)a191937429
5:3, 5:3(3)1257010362
3:5, 3:5(3)152845132
4:4, 4:4(4)37531195464895531719
ab4:4177108
Total octads38158198744931531923
Total conversion frequency (TCF) %a1.641.650.00.730.642.3
Total conversion to wild type (%)0.670.690.10.450.401.1
Total % conversion to mutanta0.900.920.260.21
Relative % of conversions to wild typea42.043.00.162.965.80.5
% postmeiotic segregationa49.351.50.448.154.92.4
Sw24.3, selfed generation 1 from the original S3 strain
8:05533
0:84542
7:14451
1:74731
6:2, 6:2(2)188176146102
2:6, 2:6(2)24725411475
5:3, 5:3(3)208199131102
3:5, 3:5(3)2262198053
4:4, 4:4(4)33164408853867431997
ab4:41110108
Total octads34061417643917032344
TCF %2.632.1023.0**1.271.075.7
Total conversion to wild type (%)1.190.9213.1**0.730.642.0
Total % conversion to mutant1.411.160.510.41
Relative % of conversions to wild type45.744.20.458.661.40.6
% postmeiotic segregation50.549.90.146.247.60.2
Sw92.1, selfed generation 1 from the original S3 strain
8:065810
0:855710
7:1421010
1:786919
6:2, 6:2(2)170177149137
2:6, 2:6(2)235187147108
5:3, 5:3(3)15215682117
3:5, 3:5(3)1961558669
4:4, 4:4(4)18567174941446016197
ab4:417301514
Total octads19360182171497316691
TCF %4.103.972.03.432.965.6
Total conversion to wild type (%)1.711.870.31.661.640.0
Total % conversion to mutant2.291.941.661.23
Relative % of conversions to wild type42.849.15.850.057.14.9
% postmeiotic segregation47.548.30.139.446.45.0
Slg60.3, selfed generation 1 from the original S2 strain
8:0713116
0:83462
7:13977
1:751587
6:2, 6:2(2)468284240221
2:6, 2:6(2)512438184160
5:3, 5:3(3)335271189190
3:5, 3:5(3)403358139119
4:4, 4:4(4)27909261182537923882
ab4:414141518
Total octads29659275242617824612
TCF %5.95.1017.2**3.052.970.3
Total conversion to wild type (%)2.742.1025.0**1.711.720.0
Total % conversion to mutant3.112.961.291.17
Relative % of conversions to wild type46.841.59.1*57.059.61.0
% postmeiotic segregation43.447.45.144.846.70.6
Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR2.3i (SG2 from S3)SR4.2i (SG2 from S2)SR1.4i (SG2 from N5)SR5.3i (SG2 from N7)
Sw67.1, a white ascospore mutation from original S3 strain
8:04344
0:82444
7:14653
1:74445
6:2, 6:2(2)735810398
2:6, 2:6(2)1261015656
5:3, 5:3(3)81499383
3:5, 3:5(3)115715454
4:4, 4:4(4)18854156302206222935
ab4:456912
Total octads19268159322239423254
TCF %2.151.902.81.481.371.0
Total conversion to wild type (%)0.840.731.30.920.811.5
Total % conversion to mutant1.281.130.530.51
Relative % of conversions to wild type39.639.20.063.561.20.3
% postmeiotic segregation50.545.02.149.749.20.0
Sg4.3, a gray ascospore mutation from original S2 strain
8:01312
0:83232
7:12341
1:71122
6:2, 6:2(2)45692524
2:6, 2:6(2)37472822
5:3, 5:3(3)43472322
3:5, 3:5(3)46572921
4:4, 4:4(4)14064174671968215132
ab4:44545
Total octads14247177011980115233
TCF %1.281.320.10.600.660.5
Total conversion to wild type (%)0.640.690.30.270.321.0
Total % conversion to mutant0.610.600.310.31
Relative % of conversions to wild type50.853.30.246.151.00.5
% postmeiotic segregation53.048.30.952.150.50.1
Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR10.2 (SG1 from S3)SR1.3 (SG1 from S2)SR1.4 (SG1 from N5)SR2.3 (SG1 from N7)
Sw17.2, a white ascospore mutation from original N5 strain
8:02454
0:83244
7:14556
1:74356
6:2, 6:2(2)56694541
2:6, 2:6(2)67843540
5:3, 5:3(3)67734940
3:5, 3:5(3)43592316
4:4, 4:4(4)23839280462681423707
ab4:450763752
Total octads24135284212702223916
TCF %1.231.322.20.770.871.7
Total conversion to wild type (%)0.530.530.10.380.380.2
Total % conversion to mutant0.480.520.250.28
Relative % of conversions to wild type52.450.50.260.858.00.3
% postmeiotic segregation56.857.60.057.257.40.0
Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR8.4 (SG1 from S3)SR2.2 (SG1 from S2)SR3.3 (SG1 from N5)SR4.1 (SG2 from N7)
Slg14.3, a light gray ascospore mutation from original N5 strain
8:04322
0:82202
7:16544
1:77433
6:2, 6:2(2)1251186663
2:6, 2:6(2)1201285561
5:3, 5:3(3)1381287258
3:5, 3:5(3)1151183834
4:4, 4:4(4)29220291852241921061
ab4:455585135
Total octads29792297492271021323
TCF %1.921.900.11.281.230.3
Total conversion to wild type (%)0.920.850.70.630.600.3
Total % conversion to mutant0.820.850.420.47
Relative % of conversions to wild type52.850.20.760.055.90.8
% postmeiotic segregation56.155.50.057.751.12.4
Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR4.2 (SG1 from S3)SR2.2 (SG1 from S2)SR4.5 (SG1 from N5)SR2.1 (SG1 from N7)
Sw41.3, a white ascospore mutation from original N5 strain
8:03422
0:83421
7:15465
1:73444
6:2, 6:2(2)137112117102
2:6, 2:6(2)1441489181
5:3, 5:3(3)15510310499
3:5, 3:5(3)1401367676
4:4, 4:4(4)26009257902340423656
ab4:463495447
Total octads26662263542386024073
TCF %2.452.145.71.911.732.2
Total conversion to wild type (%)1.130.8510.6*0.960.861.2
Total % conversion to mutant1.091.110.730.67
Relative % of conversions to wild type50.843.36.3*57.056.20.0
% postmeiotic segregation56.052.51.653.555.40.3
Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR6.3 (SG1 from S3)SR4.3 (SG1 from S2)SR1.1 (SG1 from N5)SR2.1 (SG1 from N7)
Sg16.2, a gray ascospore mutation from original N5 strain
8:03323
0:82111
7:15443
1:72131
6:2, 6:2(2)37333435
2:6, 2:6(2)67601922
5:3, 5:3(3)49444340
3:5, 3:5(3)63551514
4:4, 4:4(4)26604253942510827528
ab4:475717473
Total octads26906256682530327720
TCF %1.131.060.60.770.691.4
Total conversion to wild type (%)0.350.330.10.330.290.8
Total % conversion to mutant0.500.460.150.14
Relative % of conversions to wild type41.241.80.068.668.10.0
% postmeiotic segregation64.064.30.071.368.20.4
Octad class ( + :m)Wild type crossed to:Octad nos.Heterogeneity χ2 for the two south-slope strainsbOctad nos.Heterogeneity χ2 for the two south-slope strainsb
SR6.3 (SG1 from S3)SR4.3 (SG1 from S2)SR3.3 (SG1 from N5)SR4.1 (SG1 from N7)
Sg21.3, a gray ascospore mutation from original strain N5
8:0– 553
0:822
7:156
1:734
6:2, 6:2(2)3838
2:6, 2:6(2)2431
5:3, 5:3(3)3942
3:5, 3:5(3)2219
4:4, 4:4(4)2316524206
ab4:45759
Total octads2336024410
TCF %0.830.840.0
Total conversion to wild type (%)0.370.36
Total % conversion to mutant0.220.230.0
Relative % of conversions to wild type63.061.40.1
% postmeiotic segregation64.663.70.0
  • a For all color mutants listed, these ascal classes or conversion measures have been affected by a high frequency of spontaneous new mutations to mutant.

  • b In testing the two crosses to different SFS wild-type strains or to two different NFS wild-type strains, significant values are shown by *, P ≤ 0.0024; **, P ≤ 0.00048; no asterisk indicates homogeneity. These values are the normal ones of 0.05 and 0.01 divided by 21, the number of tests, in the Bonferroni correction for multiple tests.