TABLE 1

Names, PCR conditions, and GenBank accession numbers of microsatellite (n = 27) and gene-based markers (n = 4) genotyped in the M. nasutus × M. guttatus F2 mapping population

Marker nameAnnealing temperature (C)[MgCl2] (mm)[KCl] (mm)GenBank accession no.
Microsatellites
AAT3953°250G67654a
AAT21153°250AF012633
AAT21754°250G67645
AAT22253°250AF012634
AAT22553°250AF012635
AAT23054°450AF012636
AAT23353°250AF012637
AAT24054°350G67646
AAT24249°250AF012638
AAT26149°350G67647
AAT26555°250G67648
AAT26749°450AF012641
AAT27254°350G67649
AAT27853°3100AF012643
AAT28352°350AF012644
AAT29653°3100AF012645
AAT30055°250AF012646
AAT30855°250AF012647
AAT31251°3100AF012648
AAT33353°350G67965
AAT35052°250G67966
AAT35655°250AF012649
AAT36453°375G67650
AAT36754°350G67651
AAT37255°150G67652
AAT37453°250AF012651
AG1953°150G67653a
Gene-based markers
LFY54°2.550AF381768,a AF381769
AP357°2.550AF381766,a AF381767
CYCA55°2.550AF381762,a AF381764
CYCB55°2.550AF381763,a AF381765
  • Accession numbers refer to the sequence from the SF parent (M. nasutus) unless otherwise indicated. For gene-based markers, degenerate PCR conditions were used in the initial cloning of homologues.

  • a IM62 (M. guttatus) parent sequence.