TABLE 1

Yeast strains

StrainGenotypea
W303-1Aa
W303-1Bα
W303a/α
D111a/α GPA1/gpa1::LEU2
Tn44-1Aa sst2::LEU2
Tn44-2Aα sst2::LEU2
RAK40a sst2::HIS3
TCK13a mpt5::HIS3
TCK10a mpt5::ADE2
BXK41α mpt5::ADE2
BXK40a sst2::LEU2 mpt5::ADE2
BXK42a gpa1::LEU2 mpt5::ADE2 [pRS316-GPA1]
BXK43a gpa1::LEU2 mpt5::ADE2[pTRP-MATα]
SDK1a gpa1::URA3[pTRP-MATa]
BXK44a/α GPA1/gpa1::LEU2 mpt5::ADE2/mpt5::ADE2
BXK45a gpa1::LEU2 cln1::URA3[pTRP-MATα]
BXK46a gpa1::LEU2 cln3::URA3[pTRP-MATα]
KSK1a GPA1G302S
KSK32-7Ca GPA1G302S mpt5::HIS3
Y190a leu2-3,112 trp1-901 his3-Δ200 ade2-101 gal4Δ gal80Δ URA3::GAL-lacZ GAL-HIS3 cyhr
Y187α leu2-3,112 trp1-901 his3-Δ200 ade2-101 gal4Δ gal80Δ URA3::GAL-lacZ met
N435-1Aa his7 lys7 met6 arg1 gal4 MAL2 SUC
N435-2Aα his7 lys7 met6 arg1 gal4 MAL2 SUC
  • a With the exception of the mating testers (N435-1A and N435-2A) and two-hybrid strains (Y190 and Y187), all strains are isogenic to W303-1A and contain the markersade2-1 canl-100 ura3-1 leu2-3,112 trp1-1 his3-11,15 (diploids are homozygous for these markers) in addition to the other markers indicated.