TABLE 1

Quantitative phenotypic analysis of suppressor mutations on wild-type and clk-1 backgrounds

Length of defecation cycle (sec)
Wild typeclk-1(qm30)clk-1(e2519): 20°
Mutation15°20°25°15°20°25°
+82.8 ± 9.255.9 ± 3.442.5 ± 2.590.7 ± 6.088.3 ± 13.588.4 ± 13.877.1 ± 6.7
dsc-1(qm133)62.3 ± 9.646.9 ± 4.533.4 ± 4.076.7 ± 10.664.7 ± 12.272.9 ± 18.462.2 ± 12.9
dsc-2(qm142sd)61.1 ± 5.045.1 ± 15.531.2 ± 3.776.7 ± 21.160.0 ± 14.074.6 ± 10.058.8 ± 6.7
dec-7(qm166)55.1 ± 3.235.6 ± 3.234.1 ± 4.786.2 ± 22.258.9 ± 27.888.6 ± 13.2ND
dec-7(qm178)NDNDND91.8 ± 23.963.2 ± 24.092.3 ± 11.4ND
dsc-3(qm179)75.6 ± 4.451.9 ± 2.835.3 ± 3.576.8 ± 9.163.0 ± 6.241.6 ± 4.356.4 ± 3.0
dsc-3(qm180)74.4 ± 5.852.7 ± 2.933.7 ± 2.985.7 ± 9.268.5 ± 6.960.0 ± 20.766.7 ± 5.1
dsc-3(qm184)73.9 ± 4.753.0 ± 3.237.0 ± 2.290.3 ± 5.366.0 ± 10.353.3 ± 7.661.4 ± 5.7
dsc-4(qm182)68.1 ± 5.647.8 ± 2.533.9 ± 2.075.8 ± 7.560.7 ± 6.348.0 ± 7.760.2 ± 3.4
dsc-5(qm141)64.7 ± 8.146.3 ± 7.036.5 ± 1.879.5 ± 6.975.5 ± 9.674.8 ± 7.456.7 ± 5.1
dec(qm183)69.7 ± 5.249.9 ± 3.033.3 ± 1.2102.2 ± 11.684.8 ± 12.284.4 ± 13.983.1 ± 15.8
  • Animals were raised at 20° and scored at 15°, 20°, or 25°. The numbers given are the means ± the standard deviations of animals that had each been scored for five consecutive defecation cycles at 20° and 25° and for three consecutive cycles at 15°. The sample sizes are as follows: for N2 and clk-1(qm30) strains at 20°, n ⩾ 50; for N2 and clk-1(qm30) strains at 15° and 25°, n ⩾ 25; for ck-1(e2519) strains, n ⩾ 25. As dec-7 is tightly linked to clk-1, the defecation cycle lengths of some dec-7 strains are not determined (ND). Phenotypic analyses of the clk-1(+) dec-7(qm166) strain were carried out in the background of the dpy-17(e164) mutation, which does not affect defecation.