TABLE 1

Yeast strains used

StrainRelevant genotype
Y422MATa/MATα ade2-1/ade2-R8
Y752MATa/MATα ura3-52/ura3-52 ade2-1/ade2-R8 ime2::LEU2/ime2::LEU2
Y1094MATa/MATα ade2-1/ade2-R8 cdc28-4/cdc28-4
Y1100MATa/MATα ade2-1/ade2-R8 cdc28-4/cdc28-4 ime2Δ::URA3/ime2Δ::URA3
Y1314MATa/MATα ade2-1/ade2-R8 pCUP1-UBI-DHFRts-HA-CDC28::URA3/pCUP1-UBI-DHFRts-HA-CDC28::URA3
Y1315MATa/MATα ade2-1/ade2-R8 pCUP1-UBI-DHFRts-HA-CDC28::URA3/CDC28