TABLE 3

G4 nucleotide substitutions observed after selection at 44° (passages 51–130)

MutationGeneAA changeFirst detectedFinal statusa
125 A → GA23T → A60Fixed
1585 A → GB104bT → A60Fixed
160 C → TA34Silent80Fixed
773 G → AA239/A*27V → I100Fixed
2000 G → TD9V → L100Fixed
3916 A → GIntergenic100Fixed
5523 C → AH320N → K100Fixed
3845 C → TF415H → Y130Fixed
5570 C → AH336S → Y130Fixed
  • a These results are based on consensus and isolate sequences at passages 60, 80, 100, and 130. The status “fixed” means that all five isolates sequenced at passage 130 exhibited the change.

  • b The mutation 1585G is in a region of overlap of genes B, A, and A* but was missense only in B.