TABLE 1

Nucleotide substitutions accumulated during the high-temperature adaptation of ϕX174

MutationGeneAA changeFirst detectedFinal status
1727 C → TF242L → F4Fixed
1614 C → AF204T → N8Fixed
927 G → TJ27G → V20Polymorphic
926 G → TJ27G → C28Polymorphic
2903 G → TG170C → F4Lost