TABLE 1

Interactions of E(z)Trm with different E(z) alleles

Crossed alleles (direction of crosses indicated in parentheses)Penetrance of T1 toward T2, T3 toward T2, and A5 toward A4 transformations (%)a
T1 → T2T3 → T2A5 → A4
ap1stp1ap3stp3
Oregon-R (male)31.5077.32.841.8
n = 818n = 212n = 616n = 212n = 160
E(z)son1 (male)0**00.7**00**
n = 74n = 74n = 298n = 298n = 46
E(z)son2 (male)2**018**05**
n = 310n = 310n = 310n = 310n = 74
E(z)son3 (male)0**01**00**
n = 184n = 184n = 184n = 184n = 42
E(z)60 (male)50**07524**100**
n = 152n = 152n = 152n = 152n = 22
B2 (male) [E(z) rescue construct]2.9**01.5**01.4**
n = 854n = 854n = 854n = 854n = 214
  • apl, penetrance of weak T1 → T2 transformation indicated by the appearance of apical bristles on the first leg; stpl, penetrance of strong T1 → T2 transformation indicated by the appearance of sternopleural bristles on the proximal lateral prothorax; ap3, penetrance of weak T3 → T2 transformation indicated by the appearance of apical bristles on the third leg; stp3, penetrance of strong T3 → T2 transformation indicated by the appearance of sternopleural bristles on the metapleura. A5 toward A4 transformation was quantified by setting up a three-grade scale, grade 1 corresponding to near zero transformation, grade 2 corresponding to 50% transformed, and grade 3 corresponding to almost complete transformation of the A5 tergite toward A4 identity. By counting the number of E(z)Trm Fab-77 trans-heterozygous males of appropriate genotype with mild, medium, and strong transformations, an average percentage of tergite transformation was calculated. *, Penetrance significantly different from Oregon-R control (P < 0.05); **, penetrance highly significantly different from Oregon-R control (P < 0.001).

  • a Penetrance is percentage of the number (n) of flies examined.