TABLE 6

Microinjection of GD domain constructs and autoactivating Snake

No. embryos injected% cuticles
RNAHostDFKVDHAGastrulation
gdΔnegd9/gd9527100000Dorsalized
gd10/gd103814626280Polarized
gd2/gd2358100000Dorsalized
+/+1253532430Partly dorsalized
gdprogd2/gd2108100000Dorsalized
gd10/gd10305100000Dorsalized
+/+2384429270Partly dorsalized
gdΔne + gdprogd9/gd9390100000Dorsalized
gdvWF+/+4421529560Partly dorsalized
XaSnakegd2/gd2545100000Dorsalized
gd10/gd102854639150Polarized
  • D, dorsalized; FK, exhibits filzkorper; VD, exhibits ventral denticles; HA, hatching.