TABLE 3

Analysis of the dependence of TFIIS and Spt16 on the CTD and CTD modifying enzymes

Growth at 30°
Combined with dst1ΔCombined with spt16-197
Wild type+++++++++
rpb1Δ-110+++++++++
rpb1Δ-101++++++
rpb1Δ-103+++++
rpb1-WT(7)A5(7)+++++
rpb1-WT(9)A2(6)+++++++
ctk1Δ+++++
kin28-16+++++
srb10Δ++++++
bur1-2+++a++a+++a
  • Strains were grown in 5-FOA (dst1Δ rpb1Δ and dst1Δ kin28-16) or YPD (all other strains) media at 30° and scored as in Figure 1C. The dst1Δ rpb1Δ, dst1Δ kin28Δ, spt16-197 rpb1Δ, and spt16-197 kin28Δ strains used for plasmid shuffles were GHY496, GHY896, GHY1061, and GHY916.

  • a These results are shown for purposes of comparison and were originally reported in Murray et al. (2001).