Analysis of the effects of CTD truncation on spt4 and spt5 mutations

RPB1 (no. CTD repeats)Growth at 30°
Wild type (26)+++++++++++
rpb1Δ-110 (13)+++++++++
rpb1Δ-101 (11)++++++++
rpb1Δ-103 (10)++++++
  • Cells were grown on SC-Leu plates at 30°, replica plated to 5-FOA media, and scored for growth as in Figure 1C. Strains used were as follows: wild type, GHY498; spt4Δ, GHY627; spt5-194, GHY949; and spt5-242, GHY339.