TABLE 1

Strains

StrainGenotypeSource
FY74MATα his3Δ200 lys2-128δFred Winston
FY120MATa his4-9128 lys2-128δ leu2Δ1 ura3-52Fred Winston
FY602MATa his3Δ200 lys2-128δ leu2Δ1 trp1Δ63 ura3-52Fred Winston
GY785MATa his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 srb10Δ::TRP1Greg Prelich
GHY848MATα his4-912δ lys2-128δ suc2ΔUAS(-1900/-390) ura3-52 trp1Δ63 leu2Δ1 kin28Δ1::TRP1 [pGP457]Greg Prelich
PCY335MATa leu2 ura3 fcp1-110Karen Arndt
FY1634MATα his4-912δ lys2-128δ leu2Δ1 ura3-52 spt5-276Hartzog et al. (1998)
FY1635MATα his4-912δ lys2-128δ leu2Δ1 ura3-52 spt5-242Hartzog et al. (1998)
GHY13MATa his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt5-194
GHY166MATa his3Δ200 lys2-128δ ura3-52 leu2Δ1 trp1Δ63 spt4Δ2::HIS3
GHY339MATα his4-912δ ?his3Δ200? lys2-128δ leu2Δ1 ura3-52 spt5-242 rpb1Δ187::HIS3 [pRP112]
GHY367MATα his4-912δ lys2-128δ leu2Δ1 spt5-242
GHY368MATα his4-912δ lys2-128δ leu2Δ1 spt4Δ2::HIS3
GHY379MATα his4-912δ lys2-128δ leu2Δ1 spt5-194
GHY417MATa his4-912δ lys2-128δ leu2Δ1
GHY496MATa ?his3Δ200? his4-912δ lys2-128δ leu2Δ1 ura3-52 dst1Δ::hisG rpb1Δ187::HIS3 [pRP112]
GHY498MATa his3Δ200 his4-912δ lys2-128δ leu2Δ1 ura3-52 rpb1Δ187::HIS3 [pRP112]
GHY611MATa his4-912δ lys2-128δ leu2Δ1 trp1Δ63 SPT5-MYC
GHY617MATα his4-912δ lys2-128δ leu2Δ1 SPT5-FLAG
GHY627MATα his4-912δ lys2-128δ leu2Δ1 trp1Δ63 ura3-52 spt4Δ2::HIS3 rpb1Δ187::HIS3 ?his3Δ200? [pRP112]
GHY628MATa lys2-128δ leu2Δ1 trp1Δ63 ura3-52 spt4Δ2::HIS3 rpb1Δ187::HIS3 ?his3Δ200? [pRP112]
GHY630MATa his3Δ200 lys2-128δ ura3-52 ctk1Δ::HIS3
GHY665MATa his3Δ200 his4-912δ Iys2-12S8 ura3-52 leu2Δ1 rpb1Δ187::HIS3 ctk1Δ::HIS3 [pRP112]
GHY681MATa ?his3Δ200? lys2-128δ ura3-52 leu2Δ1 trp1Δ63 SPT5-FLAG rpb1Δ187::HIS3 [pRP112]
GHY690MATα his3Δ200 lys2-128δ ura3-52 leu2Δ1 spt4-3 trp1Δ63 ctk1Δ::HIS3
GHY705MATα his3Δ200 lys2-128δ ura3-52 ctk1Δ::HIS3 trp1Δ63 [pRS316-CTK1]
GHY708MATa his4-912δ his3Δ200 lys2-128δ ura3-52 ctk1Δ::HIS3 leu2Δ1 SPT5-FLAG [pRS316-CTK1]
GHY715MATa his3Δ200 lys2-128δ ura3-52 ctk1Δ::HIS3 spt5-194 [pRS316-CTK1]
GHY726MATα his3Δ200 lys2-128δ ura3-52 spt5-276 ctk1Δ::HIS3
GHY745MATα his4-912δ lys2-128δ suc2ΔUAS(-1900/-390) bur 1-2 SPT5-FLAG
GHY811MATα his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt5-194 srb10Δ::TRP1 [pSRB10]
GHY813MATα his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt4Δ2::HIS3 srb10Δ::TRP1 [pSRB10]
GHY815MATα his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 SPT5-FLAG srb10Δ::TRP1 [pSRB10]
GHY845MATα his3Δ200 lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt5-242 srb10Δ::TRP1 [pSRB10]
GHY881MATa his4-912δ lys2-128δ leu2Δ1 suc2ΔUAS(-1900/-390) trp1Δ63 ura3-52 spt5-194 kin28Δ1::TRP1 [pGP457]
GHY896MATa lys2-128δ ura3-52 leu2Δ1 trp1Δ63 dst1Δ::hisG kin28Δ1::TRP1 suc2ΔUAS(-1900/-390) [pGP457]
GHY909MATa his4-912δ lys2-128δ ura3-52 leu2Δ1 trp1Δ63 spt4Δ2::HIS3 kin28Δ1::TRP1 [pGP457]
GHY916MATα his4-912δ lys2-128δ leu2Δ1 trp1Δ63 ura3-52 kin28Δ1::TRP1 spt16-197 [pGP457]
GHY938MATa his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt5-242 kin28Δ1::TRP1 [pGP457]
GHY949MATa his3Δ200 lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt5-194 rpb1Δ187::HIS3 [pRP112]
GHY950MATα his3Δ200 lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt5-194 rpb1Δ187::HIS3 [pRP112]
GHY955MATa his3Δ200 lys2-128δ leu2Δ1 ura3-52 ?trp1Δ63? kin28Δ1::TRP1 ctk1Δ::HIS3 [pGP457]
GHY1043MATa his4-912δ lys2-128δ leu2Δ1 ura3-52 trp1Δ63 SPT5-FLAG kin28Δ1::TRP1 [pGH215]
GHY1061MATα his3Δ200 lys2-128δ leu2Δ1 ura3-52 trp1Δ63 spt16-197 rpb1Δ187::HIS3 [pRP112]
OY143MATa his4-912δ lys2-128δ leu2 ura3 fcp1-110
OY152MATa his4-912δ lys2-128δ leu2Δ1 spt5-194 fcp1-110
OY155MATα his4-912δ lys2-12S8 leu2Δ1 spt4Δ2::HIS3 fcp1-110
OY157MATa his4-912δ lys2-128δ leu2Δ1 spt5-242 fcp1-110
  • The spt5 mutation in strains FY1634 and GHY726, spt5-276, causes the same single-amino-acid change as that caused by spt5-242 (J. Yamada and G. H. Hartzog, unpublished data). For the sake of simplicity, this allele is referred to as spt5-242 in the text and Figure 2. ?trp1Δ63? or ?his3Δ200? indicates that the strain may carry this mutation.