TABLE 1

S. pombe strains used in this study

StrainGenotypeSource
FY80h+ ura4-D18 leu1-32 ade6-M210Our stock
FY243h cdc19-P1 ura4-D18 leu1-32 ade6-M210Our stock
FY254h ura4-D18 leu1-32 ade6-M210 can1-1Our stock
FY421h Δchk1::ura4+ ura4-D18 leu1-32 ade6-704T. Carr
FY458h+ nuc2-663 his2 leu1-32R. West
FY527h his3-D1 ura4-D18 leu1-32 ade6-M216Our stock
FY528h+ his3-D1 ura4-D18 leu1-32 ade6-M210Our stock
FY562h cdc10-V50 ura4-D18 leu1-32 ade6-M216Our stock
FY583h+ cdc22-M45 ura4-D18 leu1-32 ade6-M216Our stock
FY584h+ cdc25-22 ura4-D18 leu1-32 ade6-M216 can1-1Our stock
FY786h cdc21-M68 ura4-D18 leu1-32 ade6-M216Our stock
FY865h Δcds1::ura4+ ura4-D18 leu1-32D. Griffiths
FY918h+ Δmcm7::his3+ his3-D1 ura4-D18 leu1-32 ade6-M210 h mcm7+ his3-D1 ura4-D18 leu1-32 ade6-M216This work
FY931h+ Δmcm7::his3+ his3-D1 ura4-D18 leu1-32 ade6-M210 p[mcm7+-ura4+ ]This work
FY962h mis5-268 ura4-D18 leu1-32 ade6-M216 can1-1Our stock
FY1021h mcm7::[mcm7HA-leu1+] his3-D1 ura4-D18 leu1-32 ade6-M210This work
FY1067h cdc23::[cdc23HA-leu1+] ura4-D18 leu1-32 ade6-M210 can1-1This work
FY1086h+ cdc23-M36 ura4-D18 leu1-32 ade6-M216Our stock
FY1092h+ orp1HA ura4-D18 leu1-32 ade6-M216Our stock
FY1105h Δrad3::ura4+ ura4-D18 leu1-32 ade6-704P. Russell
FY1199h mcm7-98 ura4-D18 leu1-32 ade6-M216This work
FY1364h+ cdc23::[cdc23HA-leu1+] orp1HA ura4-D18 leu1-32 ade6-M216This work
FY1365h mcm7-98 rad4-116 ura4-D1 leu1-32 ade6-M216This work
FY1366h mcm7-98 orp1-4 ura4-D18 leu1-32 ade6-M210This work
FY1367h mcm7-98 cdc23-M36 ura4-D18 leu1-32 ade6-M216This work
FY1368h cdc23-M36 orp1-4 ura4-D18 leu1-32 ade6-M216This work
FY1369h+ cdc23-M36 rad4-116 ura4-D18 leu1-32 ade6-M210This work
FY1370h cdc21-M68 rad4-116 ura4-D18 leu1-32 ade6-M216This work
FY1401h+ cdc23-M30 ura4-D18 leu1-32 ade6-M216Our stock
FY1444h+ cdc23-M36 Δchk1::ura4+ ura4-D1 leu1-32 ade6-704This work
FY1445h+ cdc23-M30 Δchk1::ura4+ ura4-D1 leu1-32 ade6-704This work
FY1446h cdc23-M36 Δcds1::ura4+ ura4-D18 leu1-32This work
FY1447h+ cdc23-M30 Δcds1::ura4+ ura4-D18 leu1-32This work
FY1448h+ cdc23-M36 Δrad3::ura4+ ura4-D18 leu1-32 ade6-M216This work
FY1449h+ cdc23-M30 Δrad3::ura4+ ura4-D18 leu1-32 ade6-M216This work
FY1502h mcm7-98 hsk1-1312 ura4-D18 leu1-32 ade6-M210This work
FY1503h+ mcm7-98 Δrqh1::ura4+ ura4-D1 leu1-32This work
FY1504h+ mcm7-98 cdc23-M30 ura4-D18 leu1-32 ade6-M216This work
FY1617h cdc21-M68 Δcds1::ura4+ ura4-D18 leu1-32 ade6-M210This work