TABLE 3

Summary of nucleotide differences in the bicoid 3′ UTR of Drosophila

Species comparedmel/simamel/psea
Total
No. of nucleotides compared875743
No. of nucleotide differences34248
No. of substitutions per siteb0.03990.4416
Pairing regions
No. of pairs of complementary
sites7171
No. of different pairs (WC/WC)c07
No. of different pairs (WC/WC)d35(1)e
No. of different pairs (WC/NO)f05(1)g
No. of nucleotide differences326
No. of substitutions per siteb0.02140.2099
Unpaired regions
No. of sites compared733601
No. of differences31222
No. of substitutions per siteb0.04350.5087
  • The aligned sequence data are from Parsch et al. (2000).

  • a mel, D. melanogaster; sim, D. simulans;pse, D. pseudoobscura.

  • b The expected number of substitutions was calculated by Jukes and Cantor's (1969) method.

  • c The number of pairs of complementary nucleotide sites where both species have different Watson-Crick (WC) pairs. The minimum number of nucleotide changes is two.

  • d The number of pairs of complementary nucleotide sites where one species has a WC pair and the other has a GU wobble (WO) pair. The minimum number of nucleotide differences is usually one, but see below for an exception.

  • e Out of five WC/WO differences, one requires at least two nucleotide changes between the two species, where D. melanogaster has a GU pair and D. pseudoobscura has a UA Watson-Crick pair.

  • f The number of pairs of complementary nucleotide sites where one species has a WC pair and the other does not have a WC pair or a GU wobble pair (NO pair). The minimum number of nucleotide differences is usually one, but see below for an exception.

  • g Out of five WC/NO differences, one requires at least two nucleotide changes between the two species, where D. melanogaster has an AC pair and D. pseudoobscura has a UA Watson-Crick pair.