TABLE 3

Growth conditions affect the timing of septum formation

StrainMNV-glycerolYGV
A28Length (μm)29.5 ± 8.560.5 ± 16.9
No. nuclei (mode)816
APK35Length (μm)24.8 ± 10.325.0 ± 9.8
No. nuclei (mode)84
APK56Length (μm)23.5 ± 8.322.4 ± 7.3
No. nuclei (mode)88
FRY20Length (μm)26.5 ± 10.324.1 ± 8.4
No. nuclei (mode)88
  • Conidia from the indicated strains were inoculated on coverslips and incubated at 28° for 8 hr in YGV. The modal average of the nuclear number for all strains after the initial incubation was 4 (⩾90%). After 8 hr, the coverslips were washed and shifted to either YGV or MNV-glycerol. After the shift, coverslips were processed every hour for 6 hr. Coverslips were stained with Calcofluor White and Hoechst 33258 to visualize septa and nuclei, respectively. Samples for further analysis were chosen based on the SI, which was 35–40 for all strains tested. For each strain, the length of 25 cells possessing one septum was measured and the nuclei were counted. The modal average of the nuclear number was determined for all strains, and the values represent 55–95% of the sample size. Data were subjected to the Mann-Whitney test and only the populations of A28 cells shifted to YGV and MNV-glycerol were significantly different (α = 0.001). Lengths given represent the average and the standard error of the mean.