TABLE 2

Rates and distributions of lys2ΔBgl reversion events

Rates of 1-nt deletions in N-length runs in mutant strains relative to the wild-type strainb
Lys+ ratea (×10−9)Lys+ Rate Relative to wild type4N
Genotype1N2N3N4A4C5N6NOther
Wild type1.7 (1.5–2.0)1.01.01.01.01.01.01.01.01.0
pol2-422 (15–38)136.12.52.7c376.4115.6
pol3-01510 (300–900)3007203707106823016017068
msh2Δ350 (270–470)200cc180c350c430c
pol2-4 msh2Δ3,900 (3,000–4,200)2,3002701301,2002,5002601,4006,900610
pol3-01 msh2Δ28,000 (20,000–38,000)16,00015,00013,00025,0007,50030,00047,0005,700c
pol2-4 pol3-0118,000 (5,300–28,000)11,00060,0009,00015,0002,5002,7005,7001,700c
pol2-4 pol3-01 msh2Δ8,900 (3,100–23,000)5,200c1,20012,0009,1001,50024,0009401,100
  • a Numbers in parentheses under the rates correspond to the 95% confidence intervals.

  • b The rate of −1 events in a given category was obtained by multiplying the total Lys+ rate by the proportion of total events in the category (see spectra in Figures 1 and 2). The relative rate given is the rate in the mutant strain divided by the corresponding rate in the wild-type strain.

  • c No events were observed in the mutation spectra (Figures 1 and 2), so no rate could be calculated.