TABLE 6

Rates of various base substitutions leading to Ura+ reversion

Rates for specific typesa of Ura+ revertants × 10−8
C → TC → AC → GTotal rate × 10−8Revertants sequenced
StrainAbs.bRel.cAbs.Rel.Abs.Rel.
A. Mismatch repair proficient
Wt0.5310.4110.3611.322
pol1-Y869A0.921.7<0.180.180.51.16
pol2-Y831A0.340.60.521.20.3411.27
pol3-Y708A2.140.20.33.8116.143
pol3-Y708A rev3::LEU20.30.61.63.8<0.31.96
rev3::LEU20.50.90.71.8<0.11.213
B. Mismatch repair deficient
Wt111.710.112.828
pol1-Y869A1.21.25.43.1<0.66.611
pol2-Y831A2.32.39.75.7<0.51226
pol3-Y708A797955326614023
pol3-Y708A rev3::kanMX4.64.611.46.7<1.11614
rev3::kanMX0.60.61.20.7<0.11.812
  • a Median mutation rate (Table 4) corrected for proportion of a particular mutational event among revertants sequenced.

  • b Absolute mutation rate for a particular reversion event.

  • c Mutation rate for a particular reversion event relative to the strain without DNA polymerase mutation.