TABLE 5

Rates of various frameshifts leading to His+ reversion

Rates for specific typesa of His+ revertants × 10 −8
+1 in run−2 in runOtherbTotal rate × 10−8Revertants sequenced
StrainAbs.cRel.dAbs.Rel.Abs.Rel.
A. Mismatch repair proficient
Wt0.2511.110.5511.930
pol1-Y869A5.7220.350.30.350.66.418
pol2-Y831A1.24.70.60.611.72.814
pol3-Y708A3.6142.120.30.6617
B. Mismatch repair deficient
Wt871<3.6<3.68724
pol1-Y869A6407.3<43<4364015
pol2-Y831A4705.4<20<2047023
pol3-Y708A5356.1<2245>1258026
  • a Median mutation rate (Table 4) corrected for proportion of a particular mutational event among revertants sequenced.

  • b Other mutations detected were +1 and −2 frameshifts in a sequence outside of seven AċT base pair run.

  • c Absolute mutation rate for a particular reversion event.

  • d Mutation rate for a particular reversion event relative to the strain without DNA polymerase mutation.