TABLE 4

Mutation rates in strains with mutant DNA polymerase alleles

Polymerase mutation in different genetic backgroundsMutation ratea (95% confidence limits)
Canr (×10−7)Ura+ (×10−8)His+ (×10−8)Trp+ (×10−8)Ade+ (×10−8)
A. Mismatch repair-proficient strains
Wild-type Pols4.4 (2.9–5.8)1.3 (1.2–1.7)1.9 (1.4–3.6)4.1 (2.8–9.7)1.2 (1.2–2.5)
pol1-Y869A11.5 (6.2–28)1.1 (0.8–2.2)6.4 (4.1–16)4.0 (2.1–7.4)<0.9
pol1-Y951A4.4 (3.0–6.8)1.1 (0.8–1.5)2.2 (0.9–2.3)4.9 (1.3–6.3)1.1 (0.8–3.2)
pol2-Y831A7.4 (4.6–14)1.2 (0.8–2.8)2.8 (2.3–7.2)6.1 (5.0–8.3)<0.9
pol3-Y708A28 (14–32)6.1 (5.0–7.7)6.0 (4.8–11)2.5 (2.3–6.3)2.5 (2.0–3.4)
pol3-Y708A rev3::LEU24.6 (3.4–9.5)1.9 (1.7–2.6)3.2 (1.9–6.1)2.5 (1.4–2.8)1.9 (1.3–2.9)
rev3::LEU21.4 (0.9–2.5)1.2 (1.1–2.0)2.7 (1.1–3.4)<1.2<1.2
B. Mismatch repair-deficient pms1 strains
Wild-type Pols88 (73–150)2.8 (2.4–4.1)87 (54–150)23 (16–26)0.6 (0.5–1.5)
pol1-Y869A420 (240–510)6.6 (4.3–14)640 (600–1900)500 (130–1800)16 (12–79)
pol1-Y951A87 (56–120)3.7 (2.5–4.4)130 (88–170)32 (15–47)4.9 (2.8–9.0)
pol2-Y831A340 (230–420)12 (11–19)470 (270–780)140 (57–240)48 (29–97)
pol3-Y708A370 (150–540)140 (100–260)580 (340–800)140 (110–150)110 (59–130)
pol3-Y708A rev3::kanMX330 (250–500)16 (9–36)450 (350–570)71 (52–96)18 (12–27)
rev3::kanMX68 (45–150)1.8 (1.5–3.3)85 (58–175)19 (4–46)0.7 (0.5–1.5)
  • a Median for 9–27 independent cultures.