TABLE 3

Phenotypes of yeast strains with mutant replicative DNA polymerase alleles

DNA PolPolymerase alleleResidue in Kf PolSensitivity to MMSDominant or recessiveMutator effect inChromosome stability
Growth on YPDSensitivity to HUPMS1+pms1
A. Pol region II substitutions in SL/MYPS/N motifa
αpol1-Y869AE710SlowSensitiveSensitiveRecessiveWeakStrongLow
εpol2-Y645AE710MicrocoloniesNANARecessiveNANANA
δpol3-Y613AE710NoneNANARecessiveNANANA
B. Pol region III substitutions in NS/VxYG motifa
αpol1-Y951AY766NormalNormalNormalNANoneWeak (Ade+ only)Normal
εpol2-Y831AY766NormalSlightly sensitiveNormalSemidominantNoneStrongNormal
δpol3-Y708AY766SlowSensitiveSensitiveRecessiveModerateStrong and generalbLow
  • NA, not applicable.

  • a Tyrosine that was substituted is underlined.

  • b Mutator for all five markers tested.