TABLE 2

DSB-induced mutations

Cell lineSmall deletions or insertionsaDNA capturebNo PCR productMultiple PCR products
117c1
238781
3921
4213
5576111
6543112
Total196 (77.5%)21 (8.3%)32 (12.6%)4 (1.6%)
  • Classification of mutations is based on PCR products generated as described in Figure 2.

  • a Defined by a diagnostic PCR product that is indistinguishable in size from the parental 1.5-kb product (see Figure 2). Nucleotide sequences of 27 such clones are presented in Figure 3.

  • b Defined by a PCR product that is visibly larger than the parental 1.5-kb product (see Figure 2).

  • c Number of clones recovered for each category.