TABLE 1

Recovery of TFTR clones following DSB induction

TFTR colony frequencies (× 105)b
Cell linea2 days4 days6 daysMock
1<0.31.0421.3
22.33.31381.3
3511.3
41811.7
519.30.4
6130.2
  • a Cell lines 1 through 4 each contain a single integrated copy of pYL1 (Figure 1) while cell lines 5 and 6 contain an integrated copy of only the 2.5-kb BamHI fragment of pYL1. For each cell line, 5 × 106 cells were electroporated with pCMB-I-SceI or, in the case of mock experiments, with PBS alone.

  • b Calculated by dividing the number of TFTR colonies by the number of cells plated following electroporation. Cells from lines 1 and 2 were grown for 2, 4, or 6 days after electroporation before being exposed to TFT. Data presented for all other cell lines and for all mock electroporations are from experiments in which cells were grown for 6 days after electroporation before being exposed to TFT.