TABLE 1

Stability of T-DNA transmission rate to the progeny for five putative male gametophytic mutants

T-DNA transmission (%)
LineG1G2G3G4G5
Ttd34 selfed54 42–61 (1220)49 39–58 (667)52 45–57 (2349)59 52–70 (2288)
Ttd34 × WT46 42–50 (845)50 44–56 (1790)50 40–61 (283)
ms line × Ttd341 0–5 (1598)1 0–5 (2392)0.3 0–2 (793)
Ttd38 selfed48 42–60 (1140)48 41–52 (2193)51 39–67 (2588)48 40–54 (1485)
Ttd38 × WT49 42–54 (1820)51 37–59 (1526)48 41–67 (1529)
ms line × Ttd380 (1526)0 (1350)
Ttd40 selfed49 44–54 (1733)50 42–65 (1199)49 40–57 (2340)57 53–60 (1711)
Ttd40 × WT49 38–55 (937)46 36–61 (1383)57 51–58 (135)
ms line × Ttd400.4 0–1 (1737)0.2 0–0.5 (3029)0.3 0–1 (979)
Ttd41 selfed51 45–58 (1169)55 50–61 (795)54 50–66 (1668)50 42–53 (2293)
Ttd41 × WT54 41–69 (255)50 45–52 (373)52 45–60 (785)51 46–55 (1285)
ms line × Ttd419 0–15 (742)4 0–14 (1791)1 0–5 (1961)
Ttd42 selfeda54 49–67 (2428)61 57–67 (2700)62 58–68 (2007)
Ttd42 × WT49 42–57 (1435)57 50–75 (131)
ms line × Ttd421 0–2 (2916)3 0–14 (825)
  • For each Ttd line, 6 to 12 sister plants have been analyzed in selfing and crossing at each generation. Percentage of T-DNA transmission has been calculated for each sister plant as 100 × KanR × KanR/(KanR + KanS). Mean values of these percentages are reported (underlined), as well as the lowest and highest values of transmission rate obtained. Total numbers of plantlets scored are indicated in parentheses. G, generation, see materials and methods; ms, male sterile; —, not determined.

  • a For this line, plants homozygous for the T-DNA have been found in each selfing generation (see text).