TABLE 5

PLD-catalyzed hydrolysis of internalized BODIPY-PC

SPO14 genotype% BODIPY-PAa
Sec14-independent growthSporulation
Δspo140.33 ± 0.050.45 ± 0.07
SPO143.60 ± 0.102.60 ± 0.14
spo14S-112.90 ± 0.142.20 ± 0.14
spo14S-181.35 ± 0.211.15 ± 0.07
spo14S-412.45 ± 0.203.30 ± 0.02
spo14S-552.70 ± 0.142.9 ± 0.21
  • a The percentage conversion of intracellular BODIPY-PC to BODIPY was determined as described in materials and methods. The mean values ± SD from three independent experiments are shown.