TABLE 4

Sporulation proficiency of spo14 separation-of-function alleles

StrainSPO14 genotype% sporulationa
Y2581Δspo14<0.1
Y2302SPO14 CEN71 ± 12
Y2159SPO1455 ± 12
Y2162spo14S-11 CEN<0.1
Y2163spo14S-11<0.1
Y2288spo14S-18 CEN<0.1
Y2544spo14S-18<0.1
Y2160spo14S-41 CEN<0.1
Y2161spo14S-4144 ± 6
Y2168spo14S-55 CEN5 ± 1
Y2169spo14S-5551 ± 14
  • a Percentage sporulation was determined by phase-contrast microscopy. At least 600 cells were counted from three independent cultures; standard deviations were calculated with sporulation values obtained from individual experiments.