TABLE 3

Relative specific PLD activity of separation-of-function proteins

ProteinRelative PLD specific activitya
HA-Spo141.0 ± 0.01
HA-spo14S-111.03 ± 0.03
HA-spo14S-410.69 ± 0.01
HA-spo14S-180.58 ± 0.01
HA-spo14S-550.36 ± 0.01
  • a PLD assays were performed with immunopurified proteins and vesicles containing 100 μm BODIPY-PC and 5 μm PIP2 as described in materials and methods. The mean values ± SD from three independent experiments are shown.