TABLE 1

S. cerevisiae strains used in this study

StrainGenotype
Y1694MATa his4 lys2 trp1-1 ura3-1 arg4-8 spo14::HIS3 sec14-1ts ADE2 kes1-1
MATa his4 lys2 TRP1 ura3-1 arg4-8 spo14::HIS3 sec14-1ts ade2 kes1-1
CTY102aMATa ura3-52 lys2-801 Δhis3-200 sec14-1ts pct1-2
Y2281CTY102, plus YEp352 (URA3 2μ)
Y2283CTY102, plus pKR577 (SPO14 URA3 2μ)
CTY1128bCTY102 spo14::HIS3
Y2262CTY1128, plus pUN55 (URA3 CEN4)
Y2263CTY1128, plus Yep352 (URA3 2μ)
Y2264CTY1128, plus pKR325 (SPO14 URA3 CEN4)
Y2265CTY1128, plus pKR577 (SPO14 URA3 2μ)
Y2266CTY1128, plus pME1451 (spo14S-11 URA3 CEN4)
Y2421CTY1128, plus pME1568 (HA-spo14S-18 URA3 CEN4)
Y2188CTY1128, plus pME1560 (HA-spo14S-41 URA3 CEN4)
Y2194CTY1128, plus pME1593 (HA-spo14S-55 URA3 CEN4)
CTY159aMATa ura3-52 lys2-801 Δhis3-200 sec14-1ts kes1-1
CTY1130bCTY159 spo14::HIS3
Y2274CTY1130, plus pUN55 (URA3 CEN4)
Y2275CTY1130, plus Yep352 (URA3 2μ)
Y2276CTY1130, plus pKR325 (SPO14 URA3 CEN4)
Y2277CTY1130, plus pKR577 (SPO14 URA3 2μ)
Y2278CTY1130, plus pME1451 (spo14S-11URA3 CEN4)
Y2138CTY1130, plus pME1568 (HA-spo14S-18 URA3 CEN4)
Y2178CTY1130, plus pME1560 (HA-spo14S-41 URA3 CEN4)
Y2184CTY1130, plus pME1593 (HA-spo14S-55 URA3 CEN4)
NH144cMATaleu2-k HIS4 ho::LYS2 ura3 lys2 arg4-Nsp
MATα leu2::hisG his4-x ho::LYS2 ura3 lys2 ARG4
Y2170NH144, plus pME1095 (GFP-SPO14 URA3 2μ)
Y2171NH144, plus pME1528 (GFP-spo14S-11 URA3 2μ)
Y2545NH144, plus pME1697 (GFP-spo14S-18 URA3 2μ)
Y2172NH144, plus pME1561 (GFP-spo14S-41 URA3 2μ)
Y2175NH144, plus pME1594 (GFP-spo14S-55 URA3 2μ)
Y2053NH144 but homozygous spo14::LEU2
Y2581Y2053, plus pUN55 (URA3 CEN4)
Y2302Y2053, plus pME986 (HA-SPO14 URA3 CEN4)
Y2159Y2053, plus pME1095 (GFP-SPO14 URA3 2μ)
Y2162Y2053, plus pME1531 (HA-spo14S-11 URA3 CEN4)
Y2163Y2053, plus pME1528(GFP-spo14S-11 URA3 2μ)
Y2288Y2053, plus pME1568 (HA-spo14S-18 URA3 CEN4)
Y2544Y2053, plus pME1697 (GFP-spo14S-18 URA3 2μ)
Y2160Y2053, plus pME1560 (HA-spo14S-41 URA3 CEN4)
Y2161Y2053 plus pME1561 (GFP-spo14S-41 URA3 2μ)
Y2168Y2053, plus pME1593 (HA-spo14S-55 URA3 CEN4)
Y2169Y2053, plus pME1594 (GFP-spo14S-55 URA3 2μ)
Y3420MATα leu2 sec14-1 trp1::hisG ura3 Δhis3-200 cki1::LEU2 spo14::URA3
Y3426Y3420, plus pUN15 (TRP1 CEN4)
Y3430Y3420, plus pME1761 (SPO14 TRP1 CEN4)
Y3431Y3420, plus pME1753 (spo14S-11 TRP1 CEN4)
Y3428Y3420, plus pME1781 (spo14S-18 TRP1 CEN4)
Y3427Y3420, plus pME1784 (spo14S-41 TRP1 CEN4)
Y3429Y3420, plus pME1779 (spo14S-55 TRP1 CEN4)
  • a Described in Xie et al. (1998).

  • b Obtained from V. Bankaitis.

  • c Described in Hollingsworth et al. (1995).