Tetrad analysis

IntervalStrainPDaTTNPDNPD exp.bProb.ccMd%wte
ADE2SER1 MSH4 164238834<0.0134.9
ADE2SER1 msh4 591186670.7014.241
SER1HIS3 MSH4 313121150.0714.6
SER1HIS3 msh4 68481111.005.739
CAN1URA3 MSH4 33966318135<0.0137.8
CAN1URA3 msh4 101939417171.0017.346
URA3HOM3 MSH4 34263628115<0.0140.0
URA3HOM3 msh4 96943922221.0020.050
HOM3TRP2 MSH4 706205870.7013.8
HOM3TRP2 msh4 126539516140.5914.6106
HIS4CEN3 MSH4 6826972471<0.0130.0
HIS4CEN3 msh4 157164820300.0717.257
CEN3MAT MSH4 1026369415<0.0114.0
CEN3MAT msh4 1917358380.088.359

Strains RM86 (wild type) and RM85 (msh4) were used to examine the CAN1-URA3, URA3-HOM3, HOM3-TRP2, HIS4-CEN3, and CEN3-MAT intervals. Strains RM96 (wild type) and RM95 (msh4) were used to examine all intervals except CAN1URA3 and URA3-HOM3. In wild type, spore viability was 94%, and 84% of tetrads contained four viable spores. In msh4, spore viability was 52%, and 34% of tetrads contained four viable spores.

  • a Parental ditype tetrads.

  • b NPD tetrads expected (Papazian 1952).

  • c Probability based on a chi-square test that the difference between the observed and expected numbers of NPDs is due to chance.

  • d Map distance in centimorgans.

  • e The map distance in the mutant as a percentage of the map distance in wild type.