TABLE 1

Primers used to PCR amplify EcoRI (E)/MseI (M) and NotI (N)/HpaII (HP) restriction fragments

EcoRI primersMseI primers
E+0 = GACTGCGTACCAATTCM+0 = GATGAGTCCTGAGTAA
E+0-ACGAE+0-ACGCM+0-CACAM+0-CTAG
E+0-ACGGE+0-ACGTM+0-CACGM+0-CTAC
M+0-CACCM+0-CTAT
M+0-CACT
NotI primersHpaII primers
N+0 = GACTGCGTATTGGCCGCHP+0 = GGCGAATCCTAAGCGG
N+0-AAGN+0-ATTHP+0-ATAHP+0-AAC
N+0-AGGN+0-AATHP+0-TACHP+0-CAC
N+0-ACAN+0-TGCHP+0-CAC
  • The +0 primers for each restriction fragment type are shown followed by more selective primers with plus three or plus four nucleotide extensions.