TABLE 1

Aedes aegypti cDNA genes analyzed in this study

Gene name	Accession no.	T_a	Size	Forward/reverse primer sequence	Primer location	A. aegypti linkage location	Drosophila genome no.	Drosophila physical location (chromosome no., Mb)
Chromosome 1
Hemepoly (hemolymph polypeptide)^a	U11235	60	385	CGACAAGCGCCAGCAGAAGG	39	I, 0	CG6186	1, 29
				AGTTGGCCGGGTCCACTTCG	423
LF198	T58319	48	284	CTGGCGTAGATTCCGTGCTG	52	I, 1	CG2286	1, 9
				TCCGTGTTGACGTAGGTGGC	235
Erudi (enhancer of rudimentary)^a	U66869	46	444	CGGACGAAGCTGAATGAAGA	13	I, 4	CG1871	1, 10
				TCCGCTAACTGATCCACGAA	456
Sialokin1 (salivary vasodilatory protein-sialokinin1)	AF108099	60	298	CCCGATAAATCCTTTCCTTC	83	I, 4	NS
				AAATGGGTATCCCTTTCCTG	380
Immuno (immunophilin)^a	AI618957	60	360	AAATCTCGCACCCGTAAA	22	I, 4	NS
				GCCCTCGTCAAAGTTCAG	381
Cathbp (cathepsin b-like thiol protease)^a	L41940	60	343	CAAATTCGGAACCTCACCAG	133	I, 7	CG10992	1, 15
				TATCCACCCTTGCATCCATC	475
LF090	T58320	48	145	AGCAGAATGGCTCCCCGTAA	31	I, 13^b	CG1527	1, 9
				ATGGTTTCCTTGCCGGACAG	175
VitgRecp (vitellogenin receptor)	AI650188	60	308	CCTCTGCAAGAAGCCGATGT	246	I, 13	NS
				ACCCAGTCGTGGCTGTTGAT	553
Transfer (transferrin precursor)	AF019117	60	309	ATGCGGCCATCCAGGTTCAG	146	I, 14	CG6186	1, 20
				CCCGCCGACTTCAGTTTCGT	454
Allatod, Allatode (allatotropin), Rf1-5^a	U65314	43	289	GAACGGATGCTAGAAGAAAG	193	II, 32	NS
				TTAGAATGGGACTACCGAGA	481	I, 44
						II, 1
						I, 22
						III, 14
Hexam2 (hexamerin 2)	U86080	56	436	TTCCTGGTGAAGCAGAAACA	100	I, 25	CG6806
				TCATGCCATAGAATCCTTGC	535
White^a	U88851	59		TACCTGACSGCACTGCTGATTG		I, 30	CG4314	3L, 16
				TGATGACMGGCGGCCCAAC
Riboptl1 (Ribosomal protein L1)	AI658439	56	285	AGAATCTTGCGTTGCCGTAC	137	I, 30	CG5502	3R, 48
				GCTTCAGGGTTCACGTTGAT	421
Feilai-405 (SINE)^a, Rf6^a	AF107667	46	206	GATGTTCGACGCTCAGTTGT	13	I, 30	CG5409	2R, 39
				GTTGGTGATGATACCGTGCT	218	II, 19
Aamy2 (α-amylase 2)	U01208	60	330	ATGACGTTGGAGTGCGAATC	40	I, 32	CG18640	2R, 39
				ACCAGGTTGCCGTAGATGAA	369
BMIOP (blood meal-induced ovarian protein)^a	U84248	60	337	TTGAAGTCGTCGTTGCTGTT	196	I, 32	CG5709	2R, 46
				CTGCCTTTGTCATGTTTGCT	532
Chitan 1 (chitinase 1)^a	AF026491	60	327	AAACTGACCTACGCCCAAAG	687	I, 33	CG9357	2R, 45
				GTCTACGCCGATGAACGAAT	1013
CG18355	AI650010	59	258	GATGCTAATGGGAAACAAAT	250	I, 35	CG18355	2L, 8
				TGATACCGTTTAAGGCAAAG	507
Peroxnc (peroxinectin)	AI657546	58	305	GCATTTCAGCAGGGTAGA	130	I, 35	CG7660	3R, 38
				AAGATCGGCAAGAACTCA	434
PPallost (preproallatostatin)	U66841	60	326	AAGAAGAAGATTACGACGAT	16	I, 36	NS
				CAATTTCCCACTATCACTTA	341
AbdA (abdominal a)	X67132	48	367	AGCGGTAGATCCTGTTGTCT	868	I, 59	CG10325	3R, 37
				AACCTCCAACTCACCTGTTC	1234
Chromosome 2
Vmem 15a1 (vitelline membrane protein 15a-1)	S54555	54	383	TCTTGGCAATCTTCGCTCTG	74	II, 0	NS
				ATCGCGTTCCGTGTCTCATA	456
Vmem15a (vitelline membrane protein 15a)	U91682	54	332	TGAGCGACGGATAGAACTAA	313	II, 5	NS
				TACGCCAGCTAGGAAATGTA	644
TrypB (trypsin–Barillas Mury)	M77814	60	339	ACGGCTACCCTCGGTCAGTT	93	II, 6	CG11529	3L, 12
				CTACCTCGCGGTCGGTAAAG	431
Fxa (FXA-directed anticoagulant precursor)	AF050133	46	200	TTAGCACCAATCCAGCCTCA	214	II, 7	NS
				TGGCACAACTGTTGGGAAGA	413
LF233	T58327	48	218	AAAGCGCCCACCTTTGCC	38	II, 9	NS
				ATCGCCCGTCAGCTTCAGTC	255
ADPATPtla, ADPATPtlb (ADP/ATP translocase)	AI657540	57	284	CTGGCGCTACTTCATGGGTA	14	II, 9	CG16944	1, 12
	AI650176			ATCGAGGTGTTCTTCGGGTC	297	II, 27
	AI650113
Aamy1 (α-amylase 1)	AF000569	60	437	GGACTTTGTACGCGAATCTG	350	II, 10	CG17876	2R, 39
				TAAGCTGGAGCATGTGGATC	786
Mle (male-less)	AI650222	59	303	GTTTGATGACCCACCAGAA	40	II, 14	CG11680	2R, 25
				ACAGACCAACGCAGAGCA	342
D7 (D7 salivary gland protein)^a	M33156	60	342	CCTAGATTTGGCCCAGTTGT	753	II, 16	NS
				ACTCGGGCTCTGATTTGGTA	1094
LF138	T58332	60	192	AATCTGTTCGACGTGGTATG	1	II, 19^b	CG7269	2L, 6
				CCGGCAATTCGGTGATGTT	192
TrypEarl (trypsin-early)	X64362	60	459	CCAACGGTGGCATCATAGTGAAAG	295	II, 19	CG3229	2L, 3
				GATCCATTGGCGAACAGTGGAGAC	753
MtATPsyn (mitochondrial ATP synthase subunit-α)	AI650137	58	251	CCACAGCCGTCGAAGAAACC	83	II, 19	CG3612	2R, 47
				CGTCACCGATGCTCAACACG	333
InsRecp (insulin receptor)^a	U72939	46	313	AGCAGCGGCAGGATCGGTAG	24	II, 19	CG3837	3R, 35
				CGCGGAACAGCAGCAGGTAC	336
CarboxA (carboxypeptidase A)^a	AF165923	54	378	TTGAATTGTAATGGGTTGAG	68	II, 20	CG17633	2L, 10
				TTATGATAGGAATCGCTTTG	445
Glusyn (glutamine synthetase)^a	AI649983	54	441	ATTCAGAGTTGTGGGATTAT	306	II, 22^b	CG1743	1, 13
				CATTAAAGCACGTTTGTTAG	746
Sin3 (transcription factor, sin3)^a	AI561370	58	454	GTATCTGTTCCTGCGGTTGC	46	II, 39	CG8815	2R, 35
				CCTGAAGTGCTGCTTCTGCT	499
Chromosome 3
Apolipo2 (apolipophorin 2)^a	AF038654	54	329	GCTGGAATCGGTCAAACTCG	24	III, 0	CG11064	4, 1
				CCGGCCTTAACTTGCTGGTA	352
Apyr1 (apyrase1)^a	L12389	54	470	GGAATGTGACGGCGGATTT	351	III, 0b	CG4837	2R, 40
				TGGATCATGCGGCTGTTTG	820
AspSyn (asparagine synthetase)^a	U84118	60	297	GGTCGAACAATGTGCGGTAT	88	III, 2	NS
				TGATTTCCTGTGCCATCAGC	384
Dynein (cytoplasmic dynein heavy chain)	AI618900	56	276	ATGGGATGCTTTGGTTACTC	110	III, 6	CG7507	3L, 5
				TACTTTCTCAGCGTTGTTCG	385
UGALS (UGALS vitellogenin)^a	U02548	60	328	AGGGCTACAATCCTGGCTAT	80	III, 7	CG3886	2R, 35
				GTATTCTGGCTGCTTGACGT	407
Hsp70 (heat-shock protein 70)	AI658418	56	342	CCCGTCCTACGTGGCGTTCA	257	III, 8	CG4264	3R, 36
				GGTGGCCTGACGTTGCGAGT	598
LF227	T58323	51	189	AAAGTTCGTCCGGTGTCCAA	43	III, 10	NS
				TCTTCTTCAGGAGAACCGTG	231
RNAhelic (ATP-dependent RNA helicase 46)^a	AI650162	58	399	TTTGACTTCATGGACCCTCC	109	III, 11	CG11107	2R, 30
				AACTGTGACGCACATTGTCG	507
Gpd-1 (glycerol-3-phosphate dehydrogenase)	AI648308	59	239	TGTTCAAATGGAGGAAATGC	132	III, 12	CG8256	2R, 38
				CACCACCGTGGATCAGCT	370
VitgConv (vitellogenin convertase)^a	L46373	60	287	TGCACAGAAGACCACCAATG	30	III, 12	CG10772	3R, 46
				TCGACTGTTCCGCTGAGTTA	316
Malt (maltase)	M30442	48	234	GGACTGGTGGGAACATGGAA	72	III, 13	CG8696	2R, 31
				CTTATCGGACAACCGCTGGA	305
Vitg (vitellogenin)^a	L41842	60	296	AGATGGCGTCTTCGGTAAAG	41	III, 15	AE003820
				AGTGAGCACGGAACCTTTGT	336
DefA1 (defensinA1)^a	AF156088	54	193	CATTTGTTTCCTGGCTCTGT	88	III, 18	CG1385	2R, 32
				GAGCAGCACAAGCACTATC	280
TrypLate (trypsin-late)	X64363	60	325	TGGCTTTGAAGTGCCCGTTGAG	44	III, 20	CG9564	2L, 9
				CAAGTTCCTTCGTTGACCGGAGTG	368
Apyr2 (apyrase2)^a	L41391	54	317	TGATTGCATCGTCGTTGATT	103	III, 37	CG1961	1, 12
				CAACTTGCGCTGTTTGTTTT	419
Monomorphic Loci
Aahr31 (steroid hormone receptor homolog aahr31)	U87543	60	384	TGGGAGGGAGAAACCAATAC	292	M	CG11823	2R, 32
				AACTCCAGCTTGCCAACAAC	675
Aahr33 (steroid hormone receptor homolog aahr33)	AF106703	60	332	AGATCCTCCGATTATTCCTA	543	M	CG11823
				TCATACCTCAAATCCCTTCT	874
ATPaseB (V-ATPase B)	AF092934	56	195	GGTGTCACGCGGCAAGTTTA	110	M	CG11154	4, 1
				AGTCGTGGCTGCGAGATGAA	304
ATPaseC (V-ATPase C)	AF008924	51	408	TTCCAGCGGGACCGAACAGT	94	M	CG3161	2R, 26
				CACCGGCATCACCGACGATA	501
Atub (α-tubulin)	AI649995	58	264	CGGTGTCCAGATCGGTAATG	137	M	CG1913	3R, 28
				ACCACGGGCGTAGTTGTTAG	400
Btub (β-tubulin)	AI657538	58	266	AACATGGAATCGACGCCACC	294	M	CG9277	2R, 41
				TTGCGGACAACGTCCAACAC	559
Carbox (carboxypeptidase)	M79452	54	403	CAAGAAGCTAATGCGAGGAT	111	M	CG4572	3R, 40
				TATCGGTGAAACTGAATCCC	513
Chitan2 (chitinase2)	AI612670	60	344	TGCGTCTATGGTGAGTTCAA	21	M	NS
				TATCGCAGCTCTTATGAGGA	364
Chymotrp (chymotrypsin)	AI618956	60	319	CCAGTTTGGCACTCGCTTCC	55	M	CG7142
				GACGGCAATGTCATCGGGAC	373
Cinnabar (kynureninehydroxylase-white)	AF040957	48	202	TGTGCGCTAAGAACTACCAT	79	M	NS
				TTTAGCTGACTACGCCCATT	280
Ddc (dopa decarboxylase)	AI638914	60	386	CGTACCGAAATGCAAGCC	113	M	CG3686	2R, 26
				GAACTCCTCTGGCAGTCCAA	498
Ecdyrecp (ecdysteroid receptor)	U02021	60	283	GACTCGCGTGGATTGAACGG	393	M	CG1765	2R, 25
				TCTGCACCAGCCGAAGAAGC	675
Ef1α (elongation factor 1α)	AI658459	56	172	AGCCCAGGAAATGGGTAAGG	206	M	CG1873	3R, 52
				CCTGCGATGTTCCGGTAATC	377
Ef2 (elongation factor 2)	AI658391	58	200	TCCGATCCTATGGTGCAGTG	158	M	CG2238	2R, 21
				CAGACGGTTGTGCTTGTTGG	357
Ferritin	L37082	56	367	AGGTGGAAGCAATACGACTG	24	M	CG2216	3R, 50
				GCGGCATACTTCAGGTAGAT	390
GST2 (glutathiones-transferase-2)	S43311	56	112	GCTTTATCACTTTCCGATGT	3	M	NS
				AATTCACAAACGGTTCTTGC	114
G3PDH (glyceraldehyde-3-phosphate dehydrogenase)	AI650112	60	218	TTCCTGTACCACCAACTGCT	332	M	CG8893	1, 17
				CTGGAATGACCTTACCGACA	540
Hexam1 (hexamerin 1)	U86079	60	294	TCCAGCATGTCCACCAGCAC	65	M	CG2559	1, 14
				AGCATGGCGACCGTTACAGC	358
Hsp71 (heat-shock protein 71)	AI658418	56	240	GCCATGCAGCGTCTGAAGGA	190	M	CG4264
				CATACCACCGGCCAGGAGAA	429
Hsp83 (heat-shock protein 83)	AI658441	56	163	ACATGGAAATCAACCCTGAC	10	M	CG1242	3L, 3
				CTTGACCATACGGTAAATGC	172
LAP (lysosomal aspartic protease)	M95187	56	437	TCGTTTGCTTGGCCGTTCTA	141	M	CG1548	2R, 30
				TGGCTTCAGCGAAGGTTTGT	577
Mucin	AF125984	60	408	GACAGCACCCACAGGCAAAT	114	M	NS
				GCTCCTTTCAACGGGACCTT	521
NucTrn4a (nuclear transcription factor 4a)	AI618954	48	418	CTGTGGCTACATACCTTCGC	344	M	NS
				TACTATCCACGGACTGCTCC	761
Odh-1 [octanol dehydrogenase (E.C.1.1.1.73.)]	AI649985	59	249	CCGAAGGCTGGTGAGGTA	190	M	CG6598	3R, 31
				CGGGCAAAGGTTGGTTTT	438
OER (ovarian ecdysteroidogenic hormone)	AI619029	48	367	AGCCATCCAGGATCAATCTC	17	M	NS
				CACCGAAGACCAACTGTGAA	383
Perox (peroxidase)	AF098717	57	433	CTACGGGTGTCGGGAAGCAA	5	M	CG4009	3R, 37
				TAGGCGCTCAGGAATGGGAC	437
Polyubq (polyubiquitin)	AI648334	58	359	AGCTCCTCATTGTGCAGTTT	155	M	CG11624	3L, 4
				CGTTATCACTTTCCGTTGGT	513
PyrCarb (pyruvate carboxylase)	L36530	56	451	CCCCGTGTCAGAATTTGGTC	117	M
				CCATCAGTTCCGGGTACGAT	567
Rdl (dieldrin resistance)	U28803	48	441	GGGGTGACCAATAAAGCAAG	97	M	CG10537	3L, 9
				TGCGTCGTCTAAATGGATGG	537
Retro (retroposon reverse transcriptase pseudogene)	U09359	58	149	ATGGTCAAATGGCAGCGTAC	127	M	NS
				CAAACTGGACATTCGGGAGA	275
Riboptl3 (ribosomal protein L3)	AI658429	56	347	ATTGCGTTGCCGTCACCAAG	307	M
				ACCGATGCAGCAGCCTTTGA	653
SGA30k (salivary gland allergen–30 kd)	AF001927	58	334	ATTCTGTCTGGTAGGCATTG	41	M	NS
				TGTCTTCAGCGTTAGCTTCT	374
SOD (superoxide dismutase)	Cloned	48	151	TGACAACACCAACGGATGCA	37	M
				CGGACAAGGAAATCTGACTG	187
Ty7 (ras-like GTPase)	R19560	54	218	GCTTGCTGGATTAGAAACTC	170	M	CG5915	3R, 44
				TAACCACTTATTGAAGGCAC	387
VCP (vitellogenic cathepsin-b-like protease)	AF127592	60	360	GCCCGCTACCAGGACAATCA	122	M	CG10992	1, 15
				CTGAAACGGCCCAGCAGGAT	481
Vmem15a2 (vitelline membrane protein 15a-2)	S54556	60	277	GTCCTCTTCACCGCCGTCAT	10	M	NS
				ATCGGGTTGGGTGGTCTGGT	286

Listed with each gene are the GenBank accession number, optimal annealing temperature (T_a) and the approximate size of the amplified product. Also listed are the sequences and locations of the oligonucleotide primers relative to the GenBank sequence, the location of the genes in the A. aegypti linkage map, the accession number of the homologue in the Drosophila Genome Project, and its physical location in the Drosophila genome.
↵^a During SSCP analysis of PCR products, we usually heat product/buffer mixtures at 95° for 5 min and plunge them in ice for an additional 5 min to allow for intrastrand complex formation before loading onto the gel. However, for the loci indicated, better resolution was obtained without heating and cooling.
↵^b These loci were not used in mapping because their genotypes failed to fit expected Mendelian ratios.