TABLE 1

The sequences of adapters, primers, and primer combinations

The sequences of EcoRI and PstI adapters
EcoRI adapter
    5′-AATTGGTACGCAGTC-3′
    5′-CTCGTAGACTGCGTACC-3′
PstI adapter
    5′-TGTACGCAGTCTTAC-3′
    5′-CTCGTAGACTGCGTACATGCA-3′
The core sequence of primers for EcoRI and PstI amplicons
EcoRI core: 5′-GACTGCGTACCAATTC-3′
PstI core: 5′-GACTGCGTACATGCAG-3′
Primer overhangs for selective amplification
Primer overhangs added to EcoRI core
    1 = AA; 2 = AG; 3 = TG; 4 = TC; 5 = CA; 6 = CT;
    7 = CG; 8 = CC; 9 = GT; 10 = GC; 11 = GG;
    12 = ACT
Primer overhangs added to PstI core
    A = ACA; B = ACC; C = AGG; D = AG;
Primer combinations used
A1 A2 A3 A4 A6 A7 A8 A9 A11
B2 B3 B4 B5 B6 B7 B8 B9 B10 B11
C4 C5 C10
D12