TABLE 4

Comparison of terpene synthase introns: number, size, placement, and phase

RcggcasHmfvet1b,cNtfeari4Ga,I>fδcadMlg-limc,dPfg-limAgg-limAgg-pin1Agfδsel2Agfδsel1AgggabiAgfEαbisTbggtaxAtglinohaCbglinohAtgg-copp1
IntronC20Atfvet1aC15Atfeari1aC15C15Atcad1aC10C10AtlimbaAtlima1a,eAtlima2a8.,eC10C10C15C15C20C15C20C10C10C20
IK51*S32*
(151)(822)
IIN76*Q56*
(96)(115)
IIIS86S84Q39S84Q37K43S8091UK84V49L39F78G93E87G34G34K107I48E108Q84
(111)(71)(ND)(91)(127)(100)(116)(87)(698)(100)(2.850)(2.734)(83)(107)(122)(120)(96)(205)(88)(283)
IVK210*Q154*Q212*K128*K123UK186*
(188)(164)(197)(912)(440)(253)
VK271**A216**T271**Q195**R189UT247**
(106)(428)(88)(92)(181)(198)
VIH344*F289*C343*G272*G266UG319*
(121)(546)(98)(108)(246)(121)
VIIY159**Y154**Y96**Y98**Y384**Y329**Y383**Y313**Q307UH359**
(278)(85)(84)(102)(87)(156)(83)(102)(181)(79)
VIIIS177*S176*P131*Y176*P125*C130*S172*183UQ171*Q143*Q129*Q167*S198*S192*S135*S137*S422*P367*S421*P352*P346UA397*
(133)(91)(ND)(115)(87)(101)(99)(ND)(826)(212)(231)(231)(83)(146)(147)(147)(88)(134)(197)(262)(129)(82)
IXE269E263E214E216E499E444E493K424M424UE473
(84)(92)(101)(98)(156)(108)(130)(55)(84)(212)
XN294**L248**Y536**Y481**Y530**L462**L462UY511**
(537)(84)(510)(96)(110)(100)(96)(357)
XIF302**T302**S255T302**S251**F256**T298**309UL303**S267**S260**S299**S336**Y327**R224**T279**R569**T514**T563**T495**E495UQ544**
(428)(98)(ND)(90)(76)(322)(103)(ND)(125)(437)(95)(95)(101)(99)(55)(472)(90)(116)(106)(140)(73)(263)
XIIA375**K376**Q328**E376**Q324**E329**E371**383UR376**E340**K334**K372**K408**K399**R295**R351**K641**R586**K633**L567**R567UD629**
(93)(86)(93)(92)(131)(103)(122)(ND)(326)(89)(174)(174)(108)(94)(142)(146)(190)(141)(99)(222)(119)(927)
XIIIA422E424R375E423R370A376E419429US423S387V380V419A455A446C342C398V688G633P680I611L611US675
(79)(248)(ND)(193)(155)(165)(1844)(ND)(91)(308)(73)(73)(102)(106)(361)(366)(82)(103)(118)(139)(82)(494)
XIVV505E507E457E507E452K459E501512UL506S467T463T502K538K529E425E481Q771Q763Q693L695UK734
(81)(87)(ND)(84)(113)(286)(583)(101)(119)(178)(402)(402)(127)(120)(76)(78)(98)(99)(124)(84)(270)
No. of Introns6666666666669989141112111114
aa601607555604550556600599603516563602637628525582868814862884870802
  • No asterisk, *, **, indicates intron inserted, respectively, between codons (does not interrupt codon); between the first and second nucleotide; between second and third nucleotide. —, intron not present; ND, intron not determined; U, intron phase not determined; intron numbers in boldface represent highly conserved introns throughout.

  • a All A. thaliana genomic sequences reported here are putative with the exception of copalyl diphosphate synthase found in the database corresponding to a published cDNA formerly called GA1 (see Table 1).

  • b Information obtained from J. Chappell (personal communication).

  • c Sequences are not complete; the authors sequenced the regions across the 5′- and 3′-splice sites (either or both) to verify the intron placement.

  • d Mlg-lim was analyzed by preparing a chimeric genome sequence utilizing cDNA from M. spicata and incomplete gDNA sequence from M. longifolia (T. Davis, unpublished data).

  • e All three Atlim A1, A2, and B sequences are putative limonene synthase homologs from the A. thaliana database that are adjacent to each other on chromosome 4 (see Table 1). Atlima1 and Atlima2 are predictions of two possible isozymes. In a recent report Atlima and Atlimb are referred to as AtTPS02 and AtTPS03 with 52 and 56% identity, respectively, to a newly defined Arabidopsis monoterpene synthase Myrcene/(E)-β-Ocimene Synthase, AtTPS10cDNA (Bohlmann et al. 2000).