TABLE 2

Two-hybrid interactions

DNA-binding-
domain hybrid
Activation-
domain hybrid
LacZ activity
LexA-ASF1GAD0.013 ± 0.008
LexA-ASF1GAD-HIR11.1 ± 0.28
LexA-ASF1(1-143)GAD0.018 ± 0.016
LexA-ASF1(1-143)GAD-HIR122 ± 3.46
LexAGAD-HIR10.047 ± 0.028
  • Values for β-galactosidase activity are the mean of three independent transformants.

  • The GAD-HIR1 fusion included amino acids 384-840 of HIR1.