TABLE 6

Deletion rates of deletion endpoint mapping plasmids

Deletion rate × 105 (C.I.) for plasmids
Relevant genotypepSTL113
(no palindrome)
orientation +
pSTL139
(no palindrome)
orientation -
pMB304
(F14C)
orientation +
pMB305
(F14S)
orientation +
pMB306
(F14C)
orientation -
sbcD+2.6 (1.7-4.3)6.5 (4.4-11)105 (29-470)7.5 (4.5-14)221(105-464)
sbcD::Tn10kan1.9 (0.4-5.4)2.3 (1.1-4.7)19 (10-42)3.8 (3.2-4.5)98 (73-139)
  • Deletion rates between tandem repeats in tetA were measured as described in materials and methods for 12-16 independent cultures grown at 37°. A 95% confidence interval (C.I.) is indicated. Replication orientation + is that of wild-type pBR322 with fork movement counter to the transcription direction of tetA. In orientation - the tetA gene has been inverted so that replication proceeds in the same direction as tetA transcription. The strains STL2172 and STL4768, sbcD+ and sbcD::Tn10, respectively, carry the additional mutations mutS::kan ΔrecA304 and are described in Table 1. The sequences of palindromic insertions are listed in Figure 2C.