TABLE 3

Genetic dependence of palindrome-stimulated deletion

Deletion rate × 10-5 (C.I.)
Genotype insertapSTL57 nonepMB302 F14CpMB303 F14S
A. 37° assays
+5.0 (1.1-6.5)230 (140-2,400)1.3 (0.3-3.9)
ΔrecA3041.1 (0.4-2.1)170 (89-210)21.8 (17-27)
sbcD::Tn10kan ΔrecA3041.2 (0.7-1.8)20 (13-31)6.7 (4.3-9.4)
B. 30° assays
ΔrecA3041.7 (0.6-7.2)310 (170-360)52 (22-200)
dnaE486 ΔrecA3041.4 (0.2-2.5)5,600 (4,100-26,950)3,000 (2,300-4,200)
dnaE486 sbcD::Tn10kan ΔrecA3043.8 (3.8-7.8)990 (370-3,200)63 (27-140)
  • Deletion rates between 101-bp tetA tandem repeats were measured as described in materials and methods for 16-64 independent cultures grown at 37° and 30° as indicated. Assays of palindrome-containing constructs were always performed in parallel with the nonpalindromic control on 2 or more days. A 95% confidence interval (C.I.) is also indicated.

  • a The sequences of palindromic insertions are listed in Figure 2C.