TABLE 1

Escherichia coli K-12 strains

StrainRelevant genotypeDerivation or source
A. Experimental strains derived from AB1157a and used in plasmid deletion assays and deletion product analysis
AB1157rec+Bachmann (1996)
JC10287Δ(srlR-recA)304Csonka and Clark (1979)
STL2172mutS201::Tn5 Δ(srlR-recA)304Lovett and Feschenko (1996)
STL2234dnaE486(ts) zae-3095::Tn10kan Δ(srlR-recA)304UVs Cys+ transductant P1 JC10287 × STL2174
STL2706dnaE925 zae::Tn10d-cam Δ(srlR-recA)304UVs Cys+ transductant P1 JC10287 × STL2704
STL4494sbcD300::Tn10kan Δ(srlR-recA)304Cys+ transductant P1 JC10287 × STL4428
STL4768sbcD300::Tn10kan mutS201::Tn5 Δ(srlR-recA)304Kmr transductant P1 STL2172 × STL4428
STL4784sbcD300::Tn10kan dnaE486(ts) Δ(srlR-recA)304Cys+ transductant P1 JC10287 × STL4475
B. Precursor strains
CAG12173cysC95::Tn10CGSCb (Singer et al. 1989)
CAG18580zae-3095::Tn10kanCGSCb (Singer et al. 1989)
E486dnaE486(ts) = polC486(ts)CGSCb (Wechsler and Gross 1971)
FG252sbcD300::Tn10kanGibson et al. (1992)
C. Intermediate precursor strains derived from AB1157a
STL672cysC95::Tn10Tcr transductant P1 CAG12173 × AB1157
STL1155dnaE486(ts) = polC486(ts) zae-3095::Tn10kanKmr Ts transductant P1 CAG18580 × E486
STL1818dnaE486(ts) zae-3095::Tn10kanKmr Ts transductant P1 STL1155 × AB1157
STL1819zae-3095::Tn10kanKms transductant P1.CAG18580 × AB1157
STL2174dnaE486(ts) zae-3095::Tn10kan cysC95::Tn10Tcr Ts transductant P1 CAG12173 × STL1818
STL2234dnaE486(ts) zae-3095::Tn10kan Δ(srlR-recA)304UVs Cys+ transductant P1 JC10287 × STL2174
STL4156sbcD300::Tn10kanKmr transductant P1 FG252 × AB1157
STL4428sbcD300::Tn10kan cysC95::Tn10Tcr transductant P1 CAG12173 × STL4156
STL4494sbcD300::Tn10kan Δ(srlR-recA)304Cys+ transductant P1 JC10287 × STL4428
STL4768sbcD300::Tn10kan mutS201::Tn5 Δ(srlR-recA)304Kmr transductant P1 STL2172 × STL4428
STL4772dnaE486(ts) cysC95::Tn10Tcr Ts transductant P1 CAG12173 × STL2453
STL4775sbcD300::Tn10kan dnaE486 cysC95::Tn10Kmr transductant P1 FG252 × STL4772
  • Phenotypes used in constructions include antibiotic resistance (Kmr, Tcr), UV sensitivity (UVs), temperature sensitivity (Ts, failure to grow at 42°), and cysteine prototrophy (Cys+).

  • a Genotype of AB1157, STL695, and their derived strains, unless otherwise indicated, includes: F- λ- hisG4 argE3 leuB6 Δ(gpt-proA)62 thr-1 thi-1 rpsL31 galK2 lacY1 ara-14 xyl-5 mtl-1 kdgK51 supE44 tsx-33 rfbD1 mgl-51 rac- qsr-.

  • b E. coli Genetic Stock Center, Yale University.