TABLE 4

Genetic interaction between gbp-2, eat-16, and egl-10

Developmental stage of newly laid eggs
(% total no. of eggs laid)
GenotypeOne-to eight-
cell
Nine-cell
comma
Post-
comma
No. of
eggs laid
Locomation rate
(body bends per min)
Unlaid eggs
per adult
Pumping rate
(pumps per min)
N2 (wild type)694018921.5 ± 1.219.0 ± 1.2230 ± 10
gpb-2(pk751)0742611418.8 ± 1.532.1 ± 1.6129 ± 8
egl-10(md176)001001342.9 ± 0.343.1 ± 1.1238 ± 8
gpb-2(pk751);egl-10(md176)0782212018.5 ± 1.731.3 ± 1.3120 ± 7
eat-16(sy438)955017639.2 ± 1.13.8 ± 0.3146 ± 6
gpb-2(pk751)eat-16(sy438)0683210917.5 ± 1.631.0 ± 1.5135 ± 6
eat-16(sy438);egl-10(md176)0386217020.5 ± 1.030.0 ± 1.1141 ± 7
  • At least 20 adult animals were assayed for each genotype (see materials and methods). Values reported are the means 6 standard errors. The eat-16(sy438) and egl-10(md176) alleles are putative loss-of-function mutations (Koelle and Horvitz 1996; HaiduCronin et al. 1999). ANOVA comparing groups for different variables was conducted. A significant effect of group was noted for locomotion (F = 75.9, P < 0.001), for unlaid eggs per adult (F = 142.5, P < 0.001), and for pharyngeal pumping (F = 44.5, P < 0.001). Differences between genotypes for the frequency of body bends during locomotion, unlaid eggs per adult, and pharyngeal pumping were analyzed using Student’s t-test. Differences from gpb-2(pk751) for the frequency of body bends during locomotion include: eat-16(sy438) (P < 0.001) and egl-10(md176) (P < 0.001). No significant difference was obtained for the frequency of body bends during locomotion between gpb-2(pk751) and N2 (wild type) (P < 0.064), gpb-2(pk751);egl-10(md176) (P = 0.44), gpb-2(pk751)eat-16(sy438) (P = 0.23), and eat-16(sy438);egl-10(md176) (P = 0.17). Differences from gpb-2(pk751) for the unlaid eggs per adult include: N2 (wild type) (P < 0.001), eat-16(sy438) (P < 0.001), and egl-10(md176) (P < 0.001). No significant difference was obtained for the amount of unlaid eggs per adult between gpb-2(pk751) and gpb-2(pk751);egl-10(md176) (P = 0.31), gpb-2(pk751)eat-16(sy438) (P = 0.30) and eat-16(sy438);egl-10(md176) (P = 0.11). The following differences were obtained for pharyngeal pumping: gpb-2(pk751) from N2 (wild type) (P < 0.001) and egl-10(md176) (P < 0.001). No significant differences were found for pharyngeal pumping between gpb-2(pk751) and gpb-2(pk751);egl-10(md176) (P = 0.18), gpb-2(pk751)eat-16(sy438) (P = 0.26), and eat-16(sy438);egl-10(md176) (P = 0.15).