TABLE 3

Genetic interactions between gpb-2, egl-30, and goa-1

Developmental stage of newly laid eggs
(% total no. of eggs laid)
GenotypeOne- to eight-
cell
Nine-cell
comma
Post-
comma
No. of
eggs laid
Locomation rate
(body bends per min)
Unlaid eggs
per adult
Pumping rate
(pumps per min)
N2 (wild type)595019718.4 ± 2.017.6 ± 0.6258 ± 12
gpb-2(pk751)0802015617.5 ± 2.130.3 ± 1.6140 ± 8
egl-30(n686)0257513110.8 ± 1.539.3 ± 1.3279 ± 12
egl-30(n686)gpb-2(pk751)0138712810.0 ± 1.237.1 ± 2.1256 ± 15
egl-30(ad805)0010067ND46.1 ± 1.9210 ± 14
egl-30(ad805)gpb-2(pk751)0010014ND39.1 ± 1.7190 ± 9
egl-30(n686)gpb-2(pk751)goa-1(pk62)0465417011.4 ± 1.040.5 ± 0.8267 ± 19
  • Twenty adult animals were assayed for each genotype to determine the developmental stage of newly laid eggs. To determine the frequency of body bends, the unlaid eggs per adult, and the pharyngeal pumping rate, 10 adult animals were assayed for each genotype (see materials and methods). Values reported are the means ± standard errors. The goa-1(pk62) allele is a putative loss-of-function mutation (Mendel et al. 1995). egl-30(ad805) is a stronger reduction-of-function mutation compared to egl-30(n686) (Trent et al. 1983; Brundage et al. 1996). Both egl-30(ad805) mutants and egl-30(ad805)gpb-2(pk751) double mutants made few tracks and were almost paralyzed when undisturbed (ND, not determined). ANOVA comparing groups for different variables was conducted. We found a significant effect of group for locomotion (F = 6.12, P < 0.001), for unlaid eggs per adult (F = 37.0, P < 0.001), and for the rate of pharyngeal pumping (F = 16.94, P < 0.001). We used a Student’s t-test to analyze the differences between genotypes. No significant differences were obtained for the frequency of body bends during locomotion between egl-30(n686) and egl-30(n686)gpb-2(pk751) (P = 0.35) and egl-30(n686)gpb-2(pk751)goa-1(pk62) (P = 0.34). The following differences were obtained for the unlaid eggs per adult: egl-30(ad805) from egl-30(ad805)gpb-2 (pk751) (P = 0.018). No significant differences were obtained for the unlaid eggs per adult between egl-30(n686) and egl-30(n686)gpb-2(pk751) (P = 0.18), and egl-30(n686)gpb-2 (pk751)goa-1(pk62) (P = 0.27). No significant differences were found for the pharyngeal pumping rate between egl-30(n686) and egl-30(n686)gpb-2(pk751) (P = 0.11) and egl-30(n686)gpb-2(pk751)goa-1(pk62) (P = 0.33), and between egl-30(ad805) and egl-30(ad805)gpb-2(pk751) (P = 0.056).