TABLE 1

Rate of egg-laying behavior of gain- and loss-of-function gpb-2 mutant animals and rescued transgenics

Time assayed
post-L4
larval stage (hr)
Developmental stage of newly laid eggs
(% total no. of eggs laid)
GenotypeTransgeneOne- to eight-
cell
Nine-cell
comma
Post-
comma
No. of
eggs laid
N2 (wild type)None296940189
gpb-2(pk751)None2907426114
gpb-2(pk751);pkIs559[gpb-2(+)]pRP2015294933197
pkIs559[gpb-2(+)]pRP2015298920204
N2 (wild type)None530946136
gpb-2(pk751)None530397104
gpb-2(pk751);pkIs559[gpb-2(+)]pRP2015530955132
pkIs559[gpb-2(+)]PRP2015530973140
  • At least 20 adult animals were assayed for each genotype (see materials and methods). Newly laid eggs were divided into three categories: one- to eight-cell stage, nine-cell to comma stage, and post-comma stage. pkIs559[gpb-2(+)] is an integrated transgene carrying multiple copies of the gpb-2 wild-type sequence that overexpress GPB-2.