TABLE 2

Details of 19 microsatellite loci used in this study

Primer
combination
Genetic
location
Cytological
location
GenBank
accession no.
Repeat
type
Expected
heterozygosity
Repeat
variance
R2
12-12.524F1-F2AC004373(AT)150.682.50.087 NS
12-17.525E6AC004721(CA)80.9617.30.053 NS
12-54.338E1-E9AC004759(CCT)110.582.60.323*
12-7652D2-D15AC004516(AC)180.9914.30.028 NS
12-97.558A4-B1AC004365(AT)170.748.40.221 NS
22-3530A3-30A6AC005889(CA)130.663.80.069 NS
22-3931A1-A3DMU12269(AAC)70.995.40.013 NS
22-4432D2-32D4AC005115(GT)120.456.60.010 NS
22-6849F9-F13DMMP20(CA)100.403.70.123 NS
22-8153D1-E2AC004641(AC)220.9924.20.163 NS
33-0.562A1-A2AC004343(TC)120.682.40.091 NS
33-16.565A6-B2AC004767(TA)140.9927.30.098 NS
33-1865D1-D3DMU14395(TC)120.996.90.597*
33-8195CDMU1951(TA)160.946.40.170 NS
43-5186E3DROPROSA(CA)6,
    (GA)12
0.737.50.156 NS
43-54.288B3DMTRXIII(CT)230.9936.50.340*
43-7393FDMU25686(AT)150.9916.20.763*
43-7794D-94DAC008193(TG)150.9621.50.499*
43-863D2-E1AC004658(AC)120.695.60.243 NS
  • Cytological and genetic locations are according to GenBank. Expected heterozygosities were calculated for all flies listed in Table 1. Significant proportions of variation explained by latitude are given after Bonferroni correction (NS, not significant; *, significant at the 0.05 level).