TABLE 3

Phenotypic distribution of events

Phenotype (%)
StrainTrp+His+ CansHis+ CanrHis-
Wild type1494 (99.6)6 (0.4)0 (<0.06)
sae2Δ458 (87.5)61 (11.7)4 (0.8)
CanrTrp- His+Trp- His-Trp+ His+Trp+ His-
Wild type23 (3.1)711 (94.7)11 (1.5)5 (0.7)
sae2Δ21 (2.8)681 (90.8)26 (3.5)22 (2.9)
  • Cells were patched on the media originally selected (SC -Trp or SC + Can) and were subsequently replicaplated to SC -His, SC +Can, and SC -Trp.