TABLE 1

Yeast strains

NameRelevant genotype or description
GRY1509MATa-inc::URA3 ade2-101 his3200 leu21 lys2Δ::hisG trp1Δ::hisG ura3-52
GRY1559MATα-inc::URA3 cyh2 his3200 leu21 lys2Δ::hisG trp1Δ::hisG ura3-52 tyr7-1
GRY1593GRY1509 + MATa-inc::mush18/21a
GRY1595GRY1559 + MATα-inc:: mush18/21
GRY1644MATa-inc::mush18/21 ade2-101 can1Δ::hisG his3200 leu21 lys2Δ::hisG trp1Δ::hisG ura3-52
GRY1650GRY1644 + pGALHO
GRY1647MATα-inc::mush18/21 can1Δ::hisG his3200 leu21 lys2Δ::hisG trp1Δ::hisG ura3-52 tyr7-1
GRY1654GRY1647 + pGALHO
yAR523GRY1654 sae2Δ::KanMX
yAR574GRY1647 sae2Δ::KanMX
yAR577GRY1647 + pCHOL
yAR579yAR574 + pCHOL
yAR581GRY1647 rad50s::URA3
yAR583yAR581 + pCHOL
yAR626GRY1647 mre11-H125N
yAR627yAR626 + pCHOL
  • a mush18/21 refers to the inverted-repeat construct that is inserted near the MAT locus (see materials and methods).