TABLE 2

Genetic modifiers of sgs1 srs2 spore lethality

StrainNo. of viable sporesNo. of inviable spores
sgs1 srs2 165 (44)213 (56)
srs1 srs2 rad51 68 (99)1 (1)
sgs1 srs2 rad52 69 (97)2 (3)
sgs1 srs2 rad57 24 (96)1 (4)
sgs1 srs2 hdf1 12 (57)9 (43)
sgs1 srs2 dnl4 11 (44)14 (56)
sgs1 srs2 dnl4 rad52 9 (100)0 (0)
sgs1 srs2 mre11 0 (0)12 (100)
sgs1 srs2 rad50 0 (0)32 (100)
sgs1 srs2 rad50 rad51 10 (100)0 (0)
sgs1 srs2 rad50 rad52 15 (100)0 (0)
sgs1 srs2 rad1 0 (0)28 (100)
sgs1 srs2 rad1 rad51 16 (94)1 (6)
sgs1 srs2 rad1 rad52 17 (94)1 (6)
sgs1 srs2 msh2 12 (39)19 (61)
sgs1 srs2 rad59 7 (14)43 (86)
sgs1 srs2 rad51 rad59 18 (95)1 (5)
sgs1 srs2 rad52 rad59 17 (100)0 (0)

All strains were constructed in the PSY316 background. Genotypes of viable spores were determined by confirmation of auxotrophic markers and/or PCR. Genotypes of inviable spores were inferred. Numbers in parentheses represent percentages.