TABLE 1

Yeast strains used in this study

StrainGenotype
W303MATa ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 rad5-535
W303RW303 MATa RDN1::ADE2 RAD5
MMY216 W303 sgs1::HIS3
MMY217 W303 srs2::LEU2
MMY218 W303 rad9::KAN
MMY219 W303 sgs1::HIS3 rad9::KAN
MMY220 W303 srs2::LEU2 rad9::KAN
MMY221 W303 sgs1::HIS3 srs2::LEU2
MMY222 W303 sgs1::HIS3 srs2::LEU2 rad9::KAN
MMY143 W303R sgs1::HIS3
MMY144 W303R sgs1::HIS3 LEU2/SIR2
MMY146 W303R sgs1::HIS3 fob1 Δ ::TRP1
MMY147 W303R sgs1::HIS3 fob1 Δ ::TRP1 LEU2/SIR2
MMY163 W303R srs2::LEU2 fob1 Δ ::URA3
PSY316aMATa ura3-52 leu2-3,112 his3-Δ200 ade2-101 lys2-801
PSY316αMATα ura3-52 leu2-3,112 his3-Δ200 ade2-101 lys2-801
PPY35 PSY316a ADE2 hmr Δ ::ADH1-GFP
PPY70 PPY35 sgs1 Δ ::hisG-URA3
PPY92 PPY35 rad1 Δ ::HIS3
PPY96 PPY35 rad51 Δ ::HIS3
PPY97 PPY35 rad57 Δ ::HIS3
PPY111 PPY35 rad52 Δ ::HIS3
PPY143PSY316α RDN1::ADE2 hmrΔ::ADH1-GFP
PPY217 PPY143 rad50 Δ ::HIS3
PPY219 PPY143 rad59 Δ HIS3
MMY249 PPY143 srs2 Δ ::LEU2
MMY257 PPY70 × MMY249
MMY258 PPY35 sgs1 Δ ::hisG-URA3 srs2 Δ ::LEU2
MMY259 PPY143 sgs1 Δ ::hisG-URA3 srs2 Δ ::LEU2
MMY282 PPY35 sgs1 Δ ::hisG-URA3 srs2 Δ ::LEU2 rad51 Δ ::HIS3
MMY283 PPY143 sgs1 Δ ::hisG-URA3 srs2 Δ ::LEU2 rad51 Δ ::HIS3
MMY291 PPY35 sgs1 Δ ::hisG-URA3 srs2 Δ ::LEU2 rad52 Δ ::HIS3
MMY292 PPY143 sgs1 Δ ::hisG-URA3 srs2 Δ ::LEU2 rad52 Δ ::HIS3
MMY313 PPY35 hdf1 Δ ::HIS3
MMY314 PPY35 dnl4 Δ ::HIS3
MMY317 PPY35 mre11 Δ ::HIS3
MMY319 PPY35 msh2 Δ ::HIS3

Strain W303R is described in Mills et al. (1999). The notation LEU2/SIR2 indicates that a single copy of the SIR2 gene has been integrated at the LEU2 locus.