TABLE 4

Fungal transformation with pLorist6Xh clones

Recipient strainaLinkage groupCosmid clonesb
his-3I4
trp-3II1
trp-1III4
amV5
pan-2VI5
qa-2VII3
  • a N. crassa strains were used as recipients. his-3, FGSC no. 455; trp-1, trp-1-21A (not at FGSC); trp-3, Lacy 693 (not at FGSC); am, deletion strain 132; pan-2, FGSC no. 4109; qa-2, FGSC no. 3957.

  • b Clones from the pLorist6Xh library used for complementation of auxotrophic strains are as follows:

    • his-3 H004E05, H004C05, H002F07, H027D09

    • trp-3 H025H09

    • trp-1 H004E07, H052C07, H084E02, H117C11

    • am H043B03, H045B07, H55B07, H109E02, H112C04

    • pan-2 H009E05, H032G09, H037H09, H073A02, H117C03

    • qa-2 H035F03, H044C04, H123E02.