TABLE 1

The number of S-clones is linearly related to chromosome size

No. of S-clones
Linkage groupSizea (Mb)Replicate 1Replicate 2Overallb
VI4.08351,3041,302
VII4.08661,0981,129
II4.61,1631,0511,161
III5.11,1981,3361,369
IV5.71,0801,7121,710
V9.21,6341,9291,903
I10.32,0641,9951,782
Total42.9
Total S-clonesc8,84010,42510,356
Total R-clonesc2,1191,9803,419
Total A-clonesc6744107
Total assigned clones11,02612,44913,882
Correlation (R)d0.960.910.87
  • a Chromosome sizes are derived from Orbach (1992).

  • b The overall statistics are computed on the addition of chromosome-clone hybridization matrices from replicates 1 and 2. For example, if in replicate 1 a clone were sorted to linkage group I and in replicate 2 a clone were sorted to linkage group II, then in the overall table it would be recorded as sorted to linkage groups I and II and be classified as an R-clone. If in replicate 1 a clone were sorted to linkage group I and in replicate 2 a clone were not to hybridize with any chromosome, then in the overall table it would be recorded as sorted to linkage group I.

  • c S-clones hybridize to only one of the chromosome-specific probes. R-clones hybridize to two or more chromosome probes, but not all probes. A-clones hybridize to every chromosome probe.

  • d The correlation is between the number of S-clones and chromosome size.