TABLE 1

Strains and plasmids

StrainGenotypeSource
1293-RMATa ade2-101 his3Δ200 leu2Δ1 lys2-801 ura3-52 trp1Δ63 ctf13-30P. Hieter
2114MATa cep3-2 ade2-101 his3Δ200 leu2Δ1 lys2-801 trp1Δ1 ura3-52 mad2::HIS3This study
2124MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289leu2-3,112 ura3-52 bar1::KANThis study
2145MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289 leu2-3,112 ura3-52 bar1::KAN (YIp URA3 cep3td)This study
2156MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289 leu2-3,112 ura3-52 bar1::KAN (YIp URA3 skp1td)This study
2158MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289leu2-3,112 ura3-52 bar1::KAN (YIp URA3 ndc10td)This study
2160MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289 leu2-3,112 ura3-52 bar1::KAN (YIp URA3 ctf13td)This study
2162MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289 leu2-3,112 ura3-52 bar1::KAN (YIp URA3 cse4td)This study
2165MATa his3Δ1 ade2-1 ade6-1 cyh2 can1 hom3-H1 trp1-289 leu2-3,112 ura3-52 bar1::KAN (YIp URA3 ctf13td) GAL10-UBR1:HIS3This study
2185MATa/MATα mif2::KAN/MIF2:LEU2 CYH2 ste5-1/ste5-1 ade5-1/+ ura3-52/+ leu2-3,112/leu2-3,112 cyh2/cyh2This study
2215MATa/MATα MIF2:LEU2 CYH2/+ ste5-1/ste5-1 ade5-1/+ ura3-52/+ leu2-3,112/leu2-3,112 cyh2/cyh2This study
2252MATa/MATα ctf13::KAN/CTF13:LEU2 CYH2 ste5-1/ste5-1 ade2-1/lys2-1/+ ura3-52/ura3-52 leu2-3,112/ leu2-3,112 cyh2/cyh2This study
2254MATa/MATα CTF13:LEU2 CYH2/+ ste5-1/ste5-1 ade5-1/+ ura3-52/+ leu2-3,112/leu2-3,112 cyh2/cyh2This study
2268MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 ctf19::KANThis study
2269MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 mcm21::KANThis study
2272MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 slk19::URA3This study
2288MATa cbf1 ura3-53 leu2-3 ade2-1 ade3-1 cyh2 his4 mad2::URA3This study
2289MATa ade2-101 his3Δ200 leu2Δ1 lys2-801 ura3-52 trp1Δ63 mck1::HIS3 mad2::URA3This study
2290-5-1MATa ade2-1 can1-100 his3 leu2 trp1 ura3 lys5 slk19::URA3 mad2::KAN1This study
2291-22-2MATa ade2-1 can1 lys5 leu2 trp1 hom3-H1 his3 ura3 mad2::KAN1 (Yip URA3 cse4td)This study
2407-5-3MATa ade2-1 hom3-H1 his3Δ1 leu2-2,113 trp1-289 ura3-52 lys2-1 ndc10-1bar1::KANThis study
2419MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 skp1-1::TRP1 skp1-4::LEU2 mad2::HIS3This study
2bAS282MATa ade2-101 his3Δ200 leu2Δ1 lys2-801 trp1Δ1 ura3-52 cep3-2A. Strunnikov
792-1-1MATa ade2-1 his3Δ1 leu2-3,112 trp1-289 ura3-52 ctf13-30 mad2-1This study
A364AMATa ade1-1 ade2-1 tyr1-H1 lys2-1 his7-H1 ura1-H1L. Hartwell
MS1357MATα trp1Δ1 ura3-52 leu2-3,112 kar3-1M. Rose
R95-1-1-1MATa cbf1Δ ura3-52 leu2-3,112 ade2-1 ade3-1 cyh2 his4R. Baker
W303aMATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1R. Rothstein
YPH1161MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 skp1-1::TRP1 skp1-4::LEU2 CFIII (CEN3.L.YPH983)P. Hieter
YPH636MATa ade2-101 his3Δ200 leu2Δ1 lys2-801 ura3-52 trp1Δ63 mck1::HIS3P. Hieter
PlasmidPurposeSource
pΔsdb24:URA3 slk19::URA3 Joe Donovan
pDB110YIp LEU2 CYH2This study
pDB112YIp CTF13 LEU2 CYH2This study
pDB117mif2 3′Δ in pPW66RThis study
pDB118cep3 3′Δ in pPW66RThis study
pDB119cse4 3′Δ in pPW66RThis study
pDB120skp1 3′Δ in pPW66RThis study
pDB130ndc10 3′Δ in pPW66RThis study
pDB131ctf13 3′Δ in pPW66RThis study
pDB133YIp MIF2 LEU2 CYH2This study
pPW66RYIp URA3 degron vector plasmidA. Varshavsky
pRC10.1mad2Δ in BluescriptR. Li