TABLE 2

Features of ptnD/+ and ptn/ptn germ-line chimeras

Genotype of embryosTypes and nos. of
germ-line chimeras
DonorHost
A. ptnD/+ germ-line cells surrounded by normal soma
ptnD/ve h thovoD1/+ptnD/ve h th: 6
ve h th/TM3ovoD1/+ve h th/TM3: 10
B. Normal germ-line cells surrounded by ptnD/+ somaa
+/+ptnD/ve h thptnD/ve h th: 3b
+/+ve h th/TM3ve h th/TM3: 9c
C. ptn/ptn germ-line cells surrounded by normal soma
ptnP893/2/TM6bovoD1/+6
ptnP890/4/TM3ovoD1/+2
TM3/TM6bovoD1/+4
ptnP893/2/ptnP890/4ovoD1/+5d
  • In A the donor and in B the host embryos derived from a cross between wm4h/wm4h; ve h th/ve h th females and wm4h/Y; ptnD/TM3 males. Pole cells of the donor embryos in A and C were transplanted into host embryos that derived from a cross of wild-type (Canton-S) females and ovoD1/Y males. In C donor embryos derived from a cross of y w/y w; ptnP890/4/TM6b, Tb females with y w/Y; ptnP893/2/TM3, Sb Ser males. Pole cells of the donor embryos were transplanted into host embryos that derived from a cross of wild-type females with ovoD1/Y males. Eclosing females were mated with y w/Y males.

  • a The donor embryos in B derived from a cross of fs(1)K10 w/ClB females with fs(1)K10 w/Y males. Their genotypes are fs(1)K10 w/fs(1)K10 w and ClB/fs(1)K10 w.

  • b All embryos received K10 w/ClB pole cells from which progeny developed. Eggs of the ptnD/ve h th host females did not hatch.

  • c Besides their own eggs six of the ve h th/TM3 host females deposited K10 eggs and three produced offspring from K10 w/ClB pole cells (besides their own progeny).

  • d Chimeras with ptnP893/2/ptnP890/4 germ-line cells did not lay eggs. Their egg primordia were similar to those that develop in ptnD/Df(3L)AC1 females.