TABLE 6

Effects of tup11Δ tup12Δ and php5Δ deletions on fbp1 promoter variants

β-Galactosidase activity
StrainRelevant genotypePromoterRepressedDerepressed
FWP112Wild typefbp1+9 ± 11586 ± 83
LAN6PWild typefbp1 (Δ-429 to -179)18 ± 1916 ± 12
RJP57tup11Δ tup12Δfbp1 (Δ-429 to -179)528 ± 62ND
RJP80php5Δfbp1 (Δ-429 to -179)6 ± 1221 ± 27
LAN170Wild typefbp1 (Δ-1399 to -336)17 ± 0559 ± 19
RJP55tup11Δ tup12Δfbp1 (Δ-1399 to -336)441 ± 15ND
RJP82php5Δfbp1 (Δ-1399 to -336)2 ± 09 ± 0
  • β-Galactosidase activity was determined from two to four independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram of soluble protein. The fbp1+ promoter contains sequences from -1508 to +284 relative to the fbp1 transcriptional start site. ND, not determined.